Question

41. points) a) Draw the catalytic triad as it appears in the active site of any serine protease: b) The following hexapeptide was cut with trypsin. thr-val-glu-lys-his-gin Using the single letter amino acid code, write the sequence of the ping pong reaction in the boxes provided below. Enzyme -Enzyme Double-displacement reaction c) You mutate the S1 pocket of trypsin from Glutamate to Isoleucine. What are the two new products of the digestion of the hexapeptide in part b (still use the single letter AA code)? 1st released: 2nd released:

Answer 1

Ser Protiane Mechanium. Catalytic towad Base Catalyst. Acid Catalyst His 57 His 57 CH2 Sey 19 Ccovalent I Calalyst) Sex 195 CH₂ Halom added to la w of sciss ile bond <Hz SOHN CN:-H -0 . CEO---HON -~ 9 HRN CHL N- C-0- CH2 Rc-N- Asp 102 Shabbies las Citis extracts it from ser to make it more Nacelophlic; ser-o attacks Carbonyl of peptide bond. - I Aspio2 Stip3: His Ht donates Tetrahedral H RN Ht to N of suissile bond Intermediate His 57 Ils 57 V Ser 195 Cll CH Ser 19 CH2 CH2 C-o-H-N Ni-#-o CO the donates Hitely Fonzyme is -H- N a CH regenerated Scanned with H-O-C=0 RN Asp 102 CSkecamScanner Acyl enzyme Intermediate RN