How do you measure the reversal potential of VG K+ channel that is closed at the...
How do you measure the reversal potential of VG K+ channel that is closed at the potassium measure using a voltage clamp? Draw the step wise steps of the voltage clam experiment and explain how it works
Homework Answers
Using an voltage clamp it is possible to measure the reverse potential of voltage gated ion channels. The pipette of voltage clamp experimental set up is attached to the K+ channel , the pipette will contain conducting particles and one electrode. another electrode will be placed on the ground. So that the change in electrode potential can be recorded using voltage clamp.
The pipette of the experiment will be of very thin diameter. The mouth of the pipette will be smooth, so that it can closely interact with the cell membrane. The suction force by the pipette will help to adhere the pipette so closely to the iron channel. The pipette will contain conducting particles and an electrode. The ion flow of the pipette connected ion channel will be only between the cystosol and pipette. The pipette act as extra cellular space. The pipette will be connected to an amplifier. The potential change inside the pipette due to iron transport can be measured using that amplifier.
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How do you measure the reversal potential of VG K+ channel that
is closed at the...
You are studying a ligand-gated ion channel. The ligand is a neurotransmitter. Upon binding the neurotransmitter,...
You are studying a ligand-gated ion channel. The ligand is a neurotransmitter. Upon binding the neurotransmitter, the ion channel opens and Na+ flows into the cell. The receptor is found on the dendrites of frog neurons. You are going to use one of the patch-clamp variations to study the effect of (1) the neurotransmitter and (2) second (and separately) the effect of an antagonist. Explain the patch-clamp technique variation you would use and why. Explain how you will study the...
could you please explain how you did each one 5. Draw a graph showing what would...
could you please explain how you did each one
5. Draw a graph showing what would happen to resting membrane potential over time, if the sodium/potassium pump were not functioning. How would this affect a neuron's ability to produce action potentials? What does this imply about the quantity of ions that normally cross the membrane during the course of an action potential? (Group D). Topic 2: Action potential conduction and synaptic transmission 6. How would you alter sodium and/or potassium...
can someone explain how action potential works using the sodium potassium pump? the steps and order...
can someone explain how action potential works using the sodium potassium pump? the steps and order of the process? when Na and K go in and out of the cell? thank you
1. You lowered a patch-clamp onto a single ion channel. How would you identify what ion...
1. You lowered a patch-clamp onto a single ion channel. How would you identify what ion species it conducts? 2. You lowered a patch-clamp onto a single ion channel, which was created from RNA that you modified from RNA for a voltage-gated sodium channel gene. Name at least one feature of that ion channel you could identify that might tell you something useful about what the piece of ion channel you modified, might do.
9. Voltage clamps are normally used to “clamp” or “freeze” membrane potential at a particular value...
9. Voltage clamps are normally used to “clamp” or “freeze”
membrane potential at a particular value and then measure the
resulting ionic currents. Explain how setting a voltage clamp at
the presynaptic terminal to a very positive potential can prevent
synaptic transmission (hint: synaptic transmission is triggered
using voltage-gated Ca++ channels, and you can assume typical Ca
concentrations inside and outside the cell from the table.
Please explain how setting a voltage clamp at the presynaptic
terminal to a very...
1. How is an increase in stimulus strength reflected by an action potential? Draw this answer...
1. How is an increase in stimulus strength reflected by an action potential? Draw this answer out and attach it to your post. 2. Why is the Nat/K* pump responsible for maintaining resting membrane potential? 3. Explain how repolarization happens. Give characteristics of the absolute and relative refractory periods in terms of voltage-gated sodium channel conformation. At what point can a new action potential be generated? 4. Outline a situation that would reduce the likelihood of an action potential occurring....
Average ! Vc= calculated measure measure measure measures 44o 423 4234,27. し601 /.ss! ı,S8 | lo....
im
not sure what fig 15 is referring to...
Average ! Vc= calculated measure measure measure measures 44o 423 4234,27. し601 /.ss! ı,S8 | lo. Y21.10.37 з 10,59 | 0,61 71.779 0.08| 0.08 | o.os | ..。3 |ル92 | 11.4/q VM -average meter voltage Vc -capacitor voltage s source voltage Calculate the voltage which should be across the capacitor at each ime constant. the calculated voltages, draw a capacitor charge curve like one in Figure 15. Using the measured capacitor...
3.2 14 marks] You are required to design, set up and physically measure the voltage gain of a BJT...
3.2 14 marks] You are required to design, set up and physically measure the voltage gain of a BJT based CE amplifier with (Emitter resistance bypassed) with Emitter Stabilized Bias scheme with an external load resistance RL (This question is independent, do not mix with question 3.1) a) Draw the schematic diagram of the set up you are contemplating for this experiment. b) To set up the experiment, what all the components (such as resistors etc.) and lab equipment are...
In answering the following questions, pretendu don't know anything about what is happening before the break...
In answering the following questions, pretendu don't know anything about what is happening before the break in the records. Keep in mind that voltage clamping is a tool that allows the experimenter to control the voltage across the membrane, to measure resultant current flows across the membrane, and from that in tomation to interpret the states of transmembrane ion channels. (So "action potential" and voltage clamp" cannot appear in a description of a single scenario; nor can "repolarization" and "voltage...
IMAGE K shows single-channel recordings from an ionotropicreceptor, that were made using the cell-attached patch-clamp...
IMAGE K shows single-channel recordings from an ionotropic
receptor, that were made using the cell-attached patch-clamp
configuration. All ion concentrations are as with normal neurons,
and ENa = +60 mV, EK = -80 mV, and ECl = -70 mV. In these traces,
the membrane potential is being held at -60 mV. In the top trace,
the experimenter stimulated the presynaptic neuron with no drug
present, and in the bottom trace they had applied a drug.
Unfortunately, the experimenter forgot which...
could you please explain how you did each one
5. Draw a graph showing what would happen to resting membrane potential over time, if the sodium/potassium pump were not functioning. How would this affect a neuron's ability to produce action potentials? What does this imply about the quantity of ions that normally cross the membrane during the course of an action potential? (Group D). Topic 2: Action potential conduction and synaptic transmission 6. How would you alter sodium and/or potassium...
9. Voltage clamps are normally used to “clamp” or “freeze”
membrane potential at a particular value and then measure the
resulting ionic currents. Explain how setting a voltage clamp at
the presynaptic terminal to a very positive potential can prevent
synaptic transmission (hint: synaptic transmission is triggered
using voltage-gated Ca++ channels, and you can assume typical Ca
concentrations inside and outside the cell from the table.
Please explain how setting a voltage clamp at the presynaptic
terminal to a very...
1. How is an increase in stimulus strength reflected by an action potential? Draw this answer out and attach it to your post. 2. Why is the Nat/K* pump responsible for maintaining resting membrane potential? 3. Explain how repolarization happens. Give characteristics of the absolute and relative refractory periods in terms of voltage-gated sodium channel conformation. At what point can a new action potential be generated? 4. Outline a situation that would reduce the likelihood of an action potential occurring....
im
not sure what fig 15 is referring to...
Average ! Vc= calculated measure measure measure measures 44o 423 4234,27. し601 /.ss! ı,S8 | lo. Y21.10.37 з 10,59 | 0,61 71.779 0.08| 0.08 | o.os | ..。3 |ル92 | 11.4/q VM -average meter voltage Vc -capacitor voltage s source voltage Calculate the voltage which should be across the capacitor at each ime constant. the calculated voltages, draw a capacitor charge curve like one in Figure 15. Using the measured capacitor...
3.2 14 marks] You are required to design, set up and physically measure the voltage gain of a BJT based CE amplifier with (Emitter resistance bypassed) with Emitter Stabilized Bias scheme with an external load resistance RL (This question is independent, do not mix with question 3.1) a) Draw the schematic diagram of the set up you are contemplating for this experiment. b) To set up the experiment, what all the components (such as resistors etc.) and lab equipment are...
In answering the following questions, pretendu don't know anything about what is happening before the break in the records. Keep in mind that voltage clamping is a tool that allows the experimenter to control the voltage across the membrane, to measure resultant current flows across the membrane, and from that in tomation to interpret the states of transmembrane ion channels. (So "action potential" and voltage clamp" cannot appear in a description of a single scenario; nor can "repolarization" and "voltage...
IMAGE K shows single-channel recordings from an ionotropic
receptor, that were made using the cell-attached patch-clamp
configuration. All ion concentrations are as with normal neurons,
and ENa = +60 mV, EK = -80 mV, and ECl = -70 mV. In these traces,
the membrane potential is being held at -60 mV. In the top trace,
the experimenter stimulated the presynaptic neuron with no drug
present, and in the bottom trace they had applied a drug.
Unfortunately, the experimenter forgot which...
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