Question

Please be detailed and very confused

4. What is the product that you expect to see after the reagent reacts with the protein samples? What is the color of this product? lm 5. How will you measure the intensity of color of the product? Im

Answer 1

Solution

Que 4 answer-

• The bradford reagent is used to measure the concentration of protein sample.
• basically dye/reagent(commasive) is protonated and acidic condition shows red colour.
• the change in commasive G-250 red colour into blue indicates presence of protein sample in given solution.
• The basic and aromatic amino acids of proteins binds with commasive reagent to make reagent-protein complex having deprotonated anionic blue form.
• blue form (reagent+protein) absorbs maximum light of 595 nm while only red reagent absorbs maximum light of 470 nm.

Que 5 answer

• By using spectrophotometer we can measure the intensity of blue colour of protein+reagent complex at 595 nm because this complex abosrobs maximum light at 595 nm wavelength.
• the intensity of blue dark or faint blue of red colour gives different absorbance value in spectrophotometer.
• According to lambert beers law concentration of sample is directly proportional to absorbance.