Question

4. Suggest a plausible arrow-pushing mechanism for the base-catalyzed reaction of the arenediazonium salt with tyrosine side chains. NO cat. base pH 9 X- NO2 imam camera modely Tyr Y TyrY H20 OH МОН NEN 5. Tryptophan can also form adducts with arenediazonium ions, although the reaction is not base- catalyzed. Suggest a plausible arrow-pushing mechanism for the reaction. NO2 L NO2 Trp Trp x x NH WEN H20 MINH NH 6. What evidence do you have that you formed covalent bonds to the protein during this reaction? 7. Would you expect protein modification with both aniline-based reagents to work ? If so, which would seen favorable to you and why? Is this consistent with what you observed?

Answer 1

4 Step! 1 X- +* Tyr | 2 . NO2 cat base PH۰۹ OH Ho Ty TN2 NEŘIn Step 2 ده كما Step3 و الملا 7:09 NEN-

NH NH ནs， གས ། དང་ N
6) a single water molecules H₂O consists of 2 hydrogen atoms bonded to 1 oxygen atom and each hydrogen shares an electron with oxygen and this oxygen shares one electron with each hydrogen.here in this solution there is free OH ions in the solution and this hydroxide ion first abstract acid proton from reactant and form phenoxide ions.

7)yes.we can develop a new protein modification strategy based on an oxidative coupling reaction and it targets electron-rich amino acids.