Question

A sample from a 25-year-old obstetric patient was referred to the hospital for antibody identification. One of the antibody screening cells was weakly positive using LISS enhancement. A panel was tested with the following results listed.

Rh MNSS P1 Lewis Lutheran Kell Duffy Kidd LISS Cell CECI CWMNS s Lea Leb Lua Lub K 1 R1R1 + + + + 2+ + 2 R1R1 3 R2R2 + 0 + 0 + + 0 0 + + k Fya Fyb Jka JK IS 37 AHG 0 + + 01+ 2+ 1+ 1+ + 100 0 + 3+ 2+ 1+ o 1+ 0 4 r'r + + + + + + OOO + + + + + + + o +00+ 0 + + + 0 0 + 0 + + 0 + + 0 0 0 0 + + + + + 0 + 0 0 0 + + + + 0 0 0 0 OOO 0 0 I too+o+o + 5 rr 6 rr 0 + 0 + + 0 + 0 + + + 0 0 + + 0 + 0 + + 0 + 0 + + + O 00+ 0 0 0 +ool + + + + + 7rr + + + + 0 3+ 0 2+ 8 rr + + 1+ 9 rr + + + + + | + + + + + 10 R1R1 Patient cells 0 0 or

What phase in the panel is showing the strongest antibody reactivity? & What does this reaction phase suggest regarding the immunoglobulin class of antibody?

Is this antibody an alloantibody or an autoantibody? or Could this antibody cross the placenta?

What is the antibody’s most likely specificity, & If the panel cells were enzyme treated and retested, what would be the expected reactions?

Is this antibody usually clinically significant? and Define clinically significant.

Answer 1

1. ANTIBODY SCREEN USE 2-3 CELLS TO DETECT IF ANTIBODY PRESENT IN SERUM

2. Basically it is used for identification of any antibody present in the serum, for transfusion purposes to match compatibility testing. or Prenatal testing for obstetric patients. in this case LISS ( loa ionic strength saline). LISS components shows there is a presence of antibody in IgM ,37 ,AHG There is no pattern found in antibodies-antibody denatured.

3. IgG can cross placenta, this is alloantibody,

4. epitope of antigen is most likely specificity of the antibody,

5. the dosage and pattern found in heterozygous or homozygous cells will clearly indicate the presence of antibodies and will further suggest about the procedure, whether it is safe or not.