Compare Sanger sequencing and NGS 1. Sanger Sequencing: Amount of DNA (high/low)? Sequence mixed DNA (Yes/No)?...

Question

Question

Compare Sanger sequencing and NGS

1. Sanger Sequencing:

Amount of DNA (high/low)?

Sequence mixed DNA (Yes/No)?

Efficiency (high/low)?

2. NGS

Amount of DNA (high/low)?

Sequence mixed DNA (Yes/No)?

Efficiency (high/low)?

Answer 1

Answer #1

Sequencing refers to the determination of sequence of DNA, RNA or protein.
DNA sequencing helps in the knowledge of the genetic code, which will further determine the expressed products and their properties.

Sanger’s method of sequencing	Next generation sequencing
It is the first sequencing method used.	It is new method used for sequencing
Sanger’s method of sequencing is also known as chain termination method. When dd NTPS are incorporated, there is no available hydroxyl group, for DNA polymerase, hence chain termination occurs at that point.	Next generation use several sequencing techniques. Different new generation or modern techniques are cumulated in the process.
The reaction is carried out in several cycles, and the incorporated dd NTPS, labeled with different dyes, give different peaks in heights. Thus, the process is time consuming. Also, more amount of DNA is required.	Next generation sequencing (NGS) uses different biotechnology methods to sequence larger sequences or entire genome is very short time (of even less than a week). Also, lesser amount DNA can be sequenced at a time.
Single type of sequence may be used	Mixed type of sequences can be used
Sangers method is less accurate	Next generation sequencing is more accurate process.

Answer 2

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