Question

1. Draw two diagrams depicting how alphaviruses and coronaviruses make their sub-genomic RNAs. Make a separate diagram for each virus family. Start your image with a diagram of genomic RNA. If possible, use color to distinguish the different types of RNA. Use arrows to depict going from one step to the next. Label the following on your diagrams:

Alphavirus; i) genomic RNA, ii) (-) sense genomic RNA, iii) internal initiation site, iv) (+) sense sub-genomic RNA, (v) polymerase, (vi) 5’ and 3’ ends.

Coronavirus; i) genomic RNA, ii) (-) sense sub-genomic RNA (only draw 1), iii) (+) sense sub-genomic RNA (only draw 1), (iv) polymerase, v) TRS, vi) Leader sequence, vii) 5’ and 3’ ends.

Your drawings should be rather simple. You really only need to label everything once, as long as its clear on your steps of what each part is. i.e. you only need to write polymerase on the diagram one time, even though you will probably show a polymerase molecule at each step. Just make your polymerase the same shape/color on each step.

Second, for the similarities and differences, the answers can be found on slides 6, 7, and 17 of last Wednesdays lecture. By describe, I mean you just need to write a single sentence describing what the difference is. Though you can write more than one sentence if you deem it necessary.

Answer 1

SOLUTION The required diagrams are : Release o Receptoa-binding 7 A Buolding 4+) RNA M Endocytosis El RNA- J Fusion (0-70 - m - NC release Genomic I RNA Insp8)+ 265 Translation Alphaviour Nasopharyngeal aspiscate RNA extraction Revenge tansouphon Real-time PCR Multiplex PCR Dara cinalysis Nucleotide degradation Sub-genomic RNA dan Coronavirus