Question

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Serine hydroxymethyltransferase is an enzyme that is found in the metabolic pathway of amino acids and catalyzes the versible conversion of L-serine to L-glycine. Answer the following questions based upon the information given about this enzyme. (28 pts) a) Draw the expected reaction of starting material and product for this reaction at physiological conditions. (6 pts) serine hydranymetiltrany erase THE NN-methylene THE glycine H2O b) A kinetic analysis of the enzyme with substrate (THF) and an unknown (to ) inhibitor provides the following Michaelis Menton plot with an Enzyme] 0.015 M. Note: K and V. values calculated using standard values (MI) 4.60E-06 4.55E-06 serine THF-inhibitor 4.50E-06 4.45E-06 0 -3.25 mM 4.40E-06 slope slope 4.35E-06 1.24E-07 2.06E-07 -THF + inhibitor 4.30E-06 intercept intercept 4.25E-06 4.26E-06 4.26E-06 4.20E-06 0 0.2 0.4 0.6 0.8 1 1.2 1.4 11S THE - Calculate V .K andk for the uninhibited reaction. Show work for full credit.(12 pts) - Calculate K, and determine the type of inhibitor. (8 pts) Type of inhibitor

Answer 1

serine hydroxymethyl transferase THE WIN-methylene THE OH H 4c-c-coo - H-c-cool NH3 Glycine Serine 6) Given : (C) = 0.015 MM LU for uninhibited reaction, valmes given in question are : slope = 1.24 x 10-7 intercept = 4.26x10-6 formula To calculate Wmax , We us the Step intercept = - - - Vmax Lisa 6 xrot - Hanya Vmax ymas = 0.23 x 109] formula To calculate Km, no use the slope: km Vmax Scanned with CamScanner

1.24 X10* - Km XING km = (0.23 x106Jx (1.24x10-7) km = 0.28 X00"] To calculate Krat, we use the formula : 1. Vmax max Riot - scas [E] 0.23x106 Keat = 0.015 I keat= 15. 33 x106 (1) for inhibited reaction, the given values in question are: Slope - 2.06x1027 [1] = 3,25 for calculating the ki, we need to know what kind of inhibition is taking place. By seeing the graph, both lines are intersecting at Y-axis which is a characteristic feature of competitive in hibition - . | toợc ko ( ) Scanned with max CamScanner

2.06 70as an inte By solving the above equation, (ki = 5] Ics Scanned with CamScanner