What are the appropriate digest conditions (buffer and temp) for BglII, HindIII, and SmaI, each individually? What conditions would you use to do a double digest with BglII AND HindIII? A double digest with HindIII AND SmaI? (Hint: Look up the enzymes and use the Double Digest Finder tool at New England Biolabs website) (2 points)
BglII
optimum temperature - 37° C
compatible buffer- Buffer O
it is not sensitive to heat inactivation
HindIII
optimum temperature - 37 ° C
compatible buffer - buffer R
it is sensitive to heat inactivation
Sma I
optimum temperature- 30 ° C
compatible buffer - buffer tango
it is sensitive to heat inactivation.
double digestion of BglII and HindIII
temperature ( ° C) | buffer | time saver | heat inactivation ( ° C) | |
BglII | 37 | NE Buffer 3.1 | yes | no |
HindIII | 37 | NE Buffer 2.1 | no | yes (80) |
double digestion of Hind III and Sma I
temperature ( ° C) | buffer | time saver | heat inactivation ( ° C) | |
HindIII | 37 | NE Buffer 2.1 | no | 80 |
SmaI | 25 | Cut smart buffer | yes | 65 |
What are the appropriate digest conditions (buffer and temp) for BglII, HindIII, and SmaI, each individually?...
. Isoschizomers are restriction enzymes that recognize the same sequence, but may not cut in the same position. You want to cut at the site G/GCGCC, but do not have SspDI. What isoschizomer could you use to cut in the same position? (Hint: Use the Enzyme Finder tool at New England Biolabs website) (1 point)
You have cut genomic DNA with AvrII and wish to ligate it into pBluescript. However, there is no AvrII site in the multiple cloning region of this plasmid. What other restriction enzyme could you use to cut the plasmid’s multiple cloning region that would produce compatible ends for ligation with your genomic DNA? (Hint: Look up the Compatible Cohesive Ends chart at New England Biolabs website and confer with pBluescript map) (1 point)
For each exercise, you can simulate the described conditions by changing the values in the Run Experiment tool of the Simulation. To be able to measure the effects on pressure or volume, slide the Rspd bar in the Properties box to either P (atm) or V (L), respectively. The slider bar for either pressure or volume will turn yellow when it is selected, and it becomes the only dependent variable, i.e., the value you measure in response to changing the...
Unit 10 8. What is the pH of a 1.0 L buffer that consists of 00750 MC,H,NH, and 0,0500 MC,HNH,Cl after the addition of 10.0 mL of 0.5 M HCl? a. Calculate moles of C,H,NH., C,H,NH,Cl, and HC b. Write a reaction of the HCl with the appropriate other species c. Set up a reaction table (will use moles and stoichiometry) using the reaction as a header. +Cl-(aq) Before Reacts After d. Looking at your "After" line, use that information...
QUESTION 1 Using the following declarations and a member of the Array class, int [ ] bArray = new int [10]; int location; Using a method in the Array class, write a statement that would change the order of the elements in the bArray array. The contents of the first cell should hold what was in the last cell. The second cell should hold what was in the next to last cell. __________________________ HINT: You must write the full statement...
please answer each question induvidually thanks
4-1 Under what conditions would it be appropriate to use a process costing system? 4- In what ways are job-order and process costing similar? 4-3 Why is cost accumulation simpler in a process costing system than it is in a job-order costing system? 4-4 How many Work in Process accounts are maintained in a company that uses process costing? 4-5 Assume that a company has two processing departments-Mixing followed by Firing. Prepare a journal...
hehe instructions: You may work on this individually or in groups. Remember you will each be responsible for knowing the material individually on exam. Please write answers on separate sheet where appropriate. This will be turned at end of class Thursday Oct. 31. credit will be based on combination of participation and attendance. You may pick up your copy of answers outside my office Friday morning and/or view key after class on Blackboard. These are good study questions for your...
In an essay of 400 words, address each element below: - What is the purpose of the ASP.NET engine? - What software packages must be installed to serve ASP.NET web pages from a computer? - What does the @Page directive do? Does it get rendered to the browser? - What is the name of the event handler you would use to have code execute each time the ASP.NET web page is loaded? - When you are adding a new Web...
please answer each one individually thanks
2-1 What is job-order costing? 2-2 What is absorption costing? 2-3 What is normal costing? 2-4 How is the unit product cost of a job calculated? 2-5 Explain the four-step process used to compute a predetermined overhead rate. 2-6 What is the purpose of the job cost sheet in a job-order costing system? 2-7 Explain why some production costs must be assigned to products through an allocation process. 2-8 Why do companies use predetermined...
What is a myocardial infarction (MI) and what are some of the major causes? Explain why they are felt to contribute to an MI. How does lifestyle play a role? Explain why it is felt they play a role. What are the signs (objective changes in the body, e.g. fever, rash, that can be detected) and symptoms (subjective changes, e.g. pain, that are personally experienced by the patient) of a myocardial infarction? How would they be different if the person...