. Some enzymes will not cut at their sequence if it is methylated, while other enzymes are not blocked by methylation. The Dam methylase of E. coli methylates adenine when in the sequence GATC, while the Dcm methylase of E. coli methylates the second cytosine in CCAGG and CCTGG sequences. Will ClaI cut a plasmid harvested from E. coli that contains the sequence ATCGATC? Explain your answer. (Hint: Look up Dam-Dcm and CpG Methylation chart at New England Biolabs website) (1 point)
Cla1 will be blocked and will not be able to cut the site ATCGATC since the Dam methylase site and Cla1 recognition sequence overlaps. Cla1 will be able to digest plasmids in ATCGATC site from a methylase mutant E.coli host.
. Some enzymes will not cut at their sequence if it is methylated, while other enzymes...
. Isoschizomers are restriction enzymes that recognize the same sequence, but may not cut in the same position. You want to cut at the site G/GCGCC, but do not have SspDI. What isoschizomer could you use to cut in the same position? (Hint: Use the Enzyme Finder tool at New England Biolabs website) (1 point)