Question

Wild type 710 green 70 dwarf 140 dwarf, curly leaves 90 green, dwarf, curly leaves 680...

Wild type 710

green 70

dwarf 140

dwarf, curly leaves 90

green, dwarf, curly leaves 680

green, curly leaves 150

Determine order and distance between genes

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Answer #1

Answer:

Wild type 710 (+ + +)

green 70 (g + +)

dwarf 140 (+ d +)

dwarf, curly leaves 90 (+ d c)

green, dwarf, curly leaves 680 (g d c)

green, curly leaves 150 (g + c)

Total = 1840

Hint: always parental combinations are more numbered than any other recombinants in linkage. Hence, parental trihybrid would be +++/gdc.

1). If single cross over occurs between g&d...

Normal : ++/gd

After cross over: + d/g +

+ d progeny= 140+90=230

g + progeny= 150+70=220

Total of this progeny= 450

Recombination frequency between g & d = (no. of recombinants/total)100

= (450/1840)100= 24.46%

2). If single cross over occurs between d and c...

Normal: ++/dc

After cross over: +c/d+

+c progeny= 150

d + progeny= 140

Total of this progeny= 290

Recombination frequency between d&c= (290/1840)100

= 15.76%

3). If single cross over occurs between g and c...

Normal: ++/gc

After cross over: +c/g+

+c progeny= 90

g + progeny= 70

Total of this progeny= 160

Recombination frequency between g&c= (160/1840)100= 8.7%

Recombination frequency (%) = Distance between genes (m.u.)

Order of genes = g------8.7m.u.-----c----15.76m.u.-------d

Gene order= g----c---d

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