Question

Create a labeled diagram and describe the gene transfer process by which the Shiga toxin gene was picked up fromShigella dysenteriae and transferred to Escherichia coli. Be as specific as possible in your description of this process. Meaning be sure to label your donor and recipient cells as the bacterial species given depending on how the genes are being transferred.

Answer 1

Answer:

The process as was follws:

• First the Shiga toxin gene is obtained from the bacterium Shigella dysenteriae.
• On the other hand, a bacteriophage H-19B (which are viruses capable of infecting bacteria) was used, extraction of the bacteriophage DNA was done and hydrolyzed with restriction enzymes (the HindII enzymes).
• Hydrolyzed bacteriophage DNA + Shiga toxin gene + ligases were used to construct a plasmid.
• The plasmid was added to a medium that already had E. coli bacteria.
• The temperature and energy source conditions were provided for E. coli to grow and Shiga toxin expression was finally obtained.

Shigella dysentenae Shigo toxin gene. Bacteriophage H-1913 V DNA extradion DNA digested with Hind 11 enzymes Shiga toxin gere DNA from H-193 Ligases & Plasmid O- Medium with E. Coli + Plasmid E. coli expressing the Shiga toxin gene E. coli=0

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