Question

35. Bacteriophage Research: Below is an example of experimental results from a bacteriophage one-step growth curve. This experiment was conducted to compare the number of bacteriophages produced in the presence or absence of a newly identified natural remedy nicknamed "Bacteriophage-Be-Gone" that has been shown by others to reduce virus production. Please analyze the results and answer the questions below. Number of bacteriophages 30 60 180 210 240 90 120 150 time (min) a. (10 points) Please label (on the graph) which curve most likely represents (A) the control bacteriophage growth curve & which curve most likely represents the results of (B) the results of the "Bacteriophage-Be-Gone" treated growth curve. Please justify your labeling in the box below. b. (10 points) Please draw AND label two additional curves that could represent the expected growth curves of the bacterial host for both the control and the "Bacteria-Be-Gone" treated samples. Please justify why you drew each curve.

Answer 1

a. The growth curve represented by solid circles is the Growth curve of "Bacteriophage be gone" treated growth curve, and the one with solid squares represents the control bacteriophage growth curve.

In the presence of Bacteriophage be Gone, it is reported that the growth/production of virus particles is reduced. In the given graph, the circle curve has an increased lag phase and reaches a lower stationary phase as compared to the control curve. This indicates that the Bacteriophage be Gone curve is for Bacteriophage division is less permissive conditions.

b.

The bacterial culture without Bacteriophage be Gone (Control; represented with Squares) will have a lower growth rate and will not be able to grow as fast as the culture with Bacteriophage (Curve A; represented with Triangles) be gone, since the control is infested with Bacteriophage and will not be able to grow as fast.

4- 4 е в в — А 6 5 CArbitrary Units) 4 No. of Bacterial cells 3 2 1 30 60 90 20 180 180 210 240 20 300 330 Time