Question

ce%20Problem-520-Sel%205X%281%29 H Tools Add-ons Help Accessibility All changes saved in Drive nal text - Times New... 12 - B I U A 2- 15 5. Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu - plaque-forming units) 6. E. coli strain B can be infected by phage T2. A liquid culture of E. coll B is infected with two different mutant T2 viruses: hr and her. (h = host range, r-rapid lysis) When this is done in liquid culture, and then plated on a mixed lawn of E. coli B and B/2, four different kinds of plaques appear. Lawn of B+B/2 Strain B Phenotype of Plaques Number Genotype hr 460 460 har hr clear, small turbid, large turbid, small clear, large Total = 40 40 1000

Answer 1

5) Date: Answer: The number of plaques Counted on a bacterial plate are = 67. 72 Ditution - 0.1 ml of an to 5 dilution. In initial concentration of undilution plage (pfulme) = Number of colonies / diluted non & volune of dilute of sample added to plate. - 67/01 x 105 = 67x10 6 of ulme. 1000 6) a) Recombination frequenry (RF) = (no. of recombinant progenly Tota psy RF =(80 x 100 = 80%, Rf (%) = Distance b/w genes €CM). so, The distance b/w hand v genes = 8cm.

b) Experiment 42:- RF = So, the Tood 6o 7100 -6% . distance s/w h and m'gene = 6cm, Experiment # 3: . Rf = (12000 ) xwo. = 12 % ₃. Se, the distance b/w iſ and m gene= 12 cm. oi the Eder of genes re-o-oh Gene map: -.....go.....he.... GCM.....m please rate my Annoch