Question

Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from free-floating DNA in the environment Competent cell-bacterial cell wall and cytoplasmic membrane become porous and allows transforming DNA to enter the cell 2. Explain why-control E. coli cells cannot grow on L.BA+amp and L.BA+amp+X-Gal. 3. goal: Name a procedure which can replace the heat shock process and achieve the same electroportation What in the ninnee of andinn amnicillin to I RA2

number 2

Answer 1

Here, control E.coli cells are used to study transformation. These cells lack the genes which will provide them with ampicillin resistance and also genes encoding beta galactosidase that can break down X-Gal. Therefore they cannot grow on LBA+amp (plates containing ampicillin) and LBA+amp+X-Gal (plates containing ampicillin and X-Gal).

Only after successful transformation, the genes responsible for ampicillin resistance and beta galactosidase will be provided to the E.coli cells and they will be able to grow on LBA+amp and LBA+amp+X-gal.