Question

2. What is the distance between two loci that give a co-transduction frequency of 17% if you used a P1 bacteriophage that packages 2 minutes of DNA in its capsid? Note: 1 minute of DNA = 40,000 bp. A) 1.57 minutes B) 80,000 bp C) 1.18 minutes D) 35,700 bp E) 47,200 bp

Answer 1

Answer for question 2

Cotransduction frequency refers to the degree of which two markers go inherited together. It depends on the physical distance between the two markers, more closer the markers are, more will be the chances of cotransduction and therefore higher will be the frequency.

There is an inverse relationship between the distance between 2 markers and their frequency of c0transduction

Farther the two markers, lesser will be the chances of getting co inherited by generalised transduction.

It is represented by Wu equation

C= 1-(D/L)^3

C= cotransduction frequency

L= size of the co transducing fragement( in minutes or kb)

17% = 1- (D/2minutes)^3

= 0.17= 1-(D/2)^3

= 2.57- 1

= 1.57 Minutes

Therefore the correct answer will be option a

4) I think option a will be the correct answer

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