Using the PCR Thermocycler, the DNA sample was heated to what temperature?
Group of answer choices
99 Degrees Celsius
90 Degrees Celsius
95 Degrees Celsius
88 Degrees Celsius
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Question 21 pts
We also used the microcentrifuge to spin our sample of cheek cells. Why is it important to balance the microcentrifuge?
Group of answer choices
Running a centrifuge with unbalanced load could permanently damage the centrifuge. It could also cause injury to you or someone else.
Perfect symmetry of the samples
If it isn't balanced, our sample is ruined
We could lose sample through the cap if it isn't balanced
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Question 31 pts
Why did Daisy swirl the 0.9% saline solution in her mouth for a full 30 seconds before expelling back into the cup?
Group of answer choices
To obtain as many cheek cells as possible
To kill any bacteria that could contaminate our sample
That is just how long she wanted to
That is how long the directions say
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Question 41 pts
After we microcentrifuge the sample for 1 minute, we can visibly see a small white pellet at the bottom of the tube with supernatant (liquid) above. What is that pellet?
Group of answer choices
Cheek Cells
Excess Saline
Food Particles from Mouth
Can't fool me, there was no pellet
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Question 51 pts
Daisy then removed the supernatant and resuspended the cell pellet in a new tube containing 30 uL of saline. She then added 200 uL of 5% Chelex. Why is the Chelex important?
Group of answer choices
After the cells are lysed, Chelex binds to cell materials like ions and molecules which might interfere with DNA. This allow the DNA to be extracted.
Chelex helps visualize our DNA
Chelex adds weight to the tube which helps keep the Microcentrifuge balanced
It helps stabalize the temperature in the Thermocycler
Using the PCR thermocycler, the DNA was heated to what temperature?
Polymerase chain reaction (PCR) is a molecular biology technique invented by Kary Mullis. The three basic steps of PCR is-denaturation, annealing, and extension. The first step is denaturation where the DNA sample is heated. The temperature of this step is 94 to 95-degree Celcius. In this step, the ds DNA separates into individual ss DNA. This step is crucial for the annealing of primers to each such ss DNA and further extension of it.
ANSWER-95 degree Celcius.
We also used the microcentrifuge to spin our sample of cheek cells. Why is it important to balance the microcentrifuge?
As stated the cheek cells were centrifuged in a microcentrifuge. It is important to balance the microcentrifuge because otherwise it can cause pressure in the rotor and damage it. It not only damages the machine but it also can harm the individual operating it.
ANSWER-Running a centrifuge with unbalanced load could permanently damage the centrifuge. It could also cause injury to you or someone else.
Why did Daisy swirl the 0.9% saline solution in her mouth for a full 30 seconds before expelling back into the cup?
Saline water rinse allows killing the bacteria present in the mouth. It improves oral health overall.
ANSWER-To kill any bacteria that could contaminate our sample
After we microcentrifuge the sample for 1 minute, we can visibly see a small white pellet at the bottom of the tube with supernatant (liquid) above. What is that pellet?
Centrifugation follows the principle of sedimentation. As the centrifuge rotates at a given speed the substance with higher density settles down and forms the pellet. The substance with lesser density floats above the pellet forming the supernatant. In this question, the correct answer is food particles from the mouth.
ANSWER-Food particles from the mouth.
Daisy then removed the supernatant and resuspended the cell pellet in a new tube containing 30 uL of saline. She then added 200 uL of 5% Chelex. Why is the Chelex important?
Chelex is a chelating material. It has been made by Bio-Rad. Its function is to purify compounds with the help of ion exchange
ANSWER-After the cells are lysed, Chelex binds to cell materials like ions and molecules which might interfere with DNA. This allows the DNA to be extracted.
Using the PCR Thermocycler, the DNA sample was heated to what temperature? Group of answer choices...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
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