Question

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Consider a membrane protein destined for the plasma membrane. From a hydropathy plot, you determine the protein has the following general structural elements: • • • 5 Trans-membrane domains (boxes 1-5) 6 soluble domains (wavy lines A-F) Flanking charges around trans-membrane domain 1. (A positive charge is towards the N-terminus, and a negative charge is towards the C-terminus.) You find that soluble domains B and E contain possible glycosylation sites. Ntorm in an ammo u arom Please use this information for Parts A-G. Question 19 Not yet answered Marked out of 1.0 Flag question Question text A. This protein is first inserted into the ER membrane. What is the name of the protein complex primarily responsible for this insertion? Answer: Question 20 Not yet answered Marked out of 1.0 Flag question Question text B. When the protein inserted into the ER membrane, please list all the soluble domain(s) (A-F) of the protein that will face the cytoplasm. Answer: Question 21 Not yet answered Marked out of 10 Flag question Question text C. Please type whether soluble domain B or E will be glycosylated. Answer: Question 22 Not yet answered Marked out of 1.0 Flag question Question text D. This protein is delivered to the plasma membrane through vesicular transport. After it reaches the plasma membrane, please type whether this glycosylated domain will face the cytoplasm or the extracellular space. Answer:

Answer 1

20.T complex is responsible for insertion of protein into ER membrane . In mammals , translocon is made up of three polypeptides called the sec61 complex .

21. It's a multipass transmembrane protein with with odd number of transmembrane helix so it's C-terminal will face the cytoplasm and N- terminal will face the ER lumen . So domain B , D& F will face the cytoplasm .

22. Domain E will be glycosylated.

23. Glycosylated domain will face the cytoplasm .