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Out of Oligomycin, Carbon Monoxide, DNP, and Rotenone, which will affect oxygen production in chloroplasts ?...

Out of Oligomycin, Carbon Monoxide, DNP, and Rotenone, which will affect oxygen production in chloroplasts ? Why do the others not affect oxygen production in chloroplasts? Please help me understand.

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Answer #1

Carbon monoxide is an inhibitor of cytochrome oxidase while rotenone is the inhibitor of complex 1. None of them is present in the chloroplast, hence they are not part of the electron transport chain of light reaction. So neither CO nor rotenone can affect oxygen production.

DNP is an uncoupler in mitochondria ETC which does not affect ETC but prevents the synthesis of ATP by allowing the protons diffuse from intermembrane space to mitochondrial matrix. So it also does not affect oxygen production.

oligomycin inhibits the ATP synthase embedded in the membrane of thylakoid and by doing so it can block the electron transport from PS II to NADP+ that will prevent the hydrolysis of water by PS II.

So its oligomycin which can affect oxygen production in the chloroplast.

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