A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...

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Signal Stop Start Stop Start A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-…

i) What is the topology of this protein in the cell membrane? The red segments correspond to either signal sequences or stop transfer sequences. The orange segments correspond to start transfer sequences

ii) What is the N-terminal sequence of the protein, after processing

iii) Is this a eukaryotic or prokaryotic protein? Why?

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Answer 1

Answer #1

1) topology will be multi pass protein where N terminus toward outside

2) after processing of N terminus signal peptide protein sequence get exposed which is starting with methione and could binding site for certain signal molecule

3) cetainly these proteins are of eukaryotes as addition of signal peptide and it's process required endoplasmic and Golgi complex which are present in eukaryotes.

Hope it's clear..thanks

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Answer 2

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A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...

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Which of these protein sequences is most likely to span a cell membrane? Gly-Asp-Val-Ala-Gly-Arg-Gly-Asn-Gly-Lys-Lys-Pro-Ser-Ser-Val-Arg-Ala-Leu-Ser Ile-Val-Leu-Pro-Ile-Val-Leu-Leu-Val-Phe-Leu-Cys-Leu-Gly-Val-Phe-Leu-Leu-Trp Lys-Asn-Trp-Arg-Leu-Lys-Asn-I

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What fragments will be obtained by a trypsin hydrolysis of the following octapeptide? Ala-Val-Trp-Lys-Phe-Gly-Arg-Met A) Ala-Val-Trp-Lys-Phe...

Met-Ala-Arg-Tyr-Ala-Asn-Asn-GluLys-Glu-Leu-Leu-TyrArg-Tyr-Ala-AsnPhe-Leu-Ala-Asn-Asn-Ile-Gly-Ala-AsnIle-SerIle-Asn-Thr-Glu-Arg-Glu-Ser-Thr-Glu-AspIle-Asn His-Glu-ArgPhe-Ala-Thr-His-Glu-Arg-Ser__Thr-Arg-Ile-Gly-Leu-Tyr-Cys-Glu-Arg-Ile-Asp-

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A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...

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Add Answer to: A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...

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A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...