Question

1.Carl Woese, who was a microbiologist and biophysicist was famous for defining the Archaea in 1977 phylogenetis taxonomy of 16S ribosomal RNA, a technique pioneered by Woese which revolutionized the discipline of microbiology Name FOUR (4) reasons WHY Woese used the 16s SSU of RNA for these studies. / / / / / / / / / / a. The Enterobacteriaceae beløpg to which class of Proteobacteria? b. The Enterobacteriaceae are divided into two groups: Mixed Acid Fermenters and Butanediol fermenters. Explain the difference between the two and end products formed: c. Name ONE organism from each group: Mixed acid fermenter: Butanediol fermenter

Answer 1

Ans. 1. I. rRNA is found in all organisms.

ii. It's comparatively simpler than DNA to study.

iii. He believed that rRNA was the most ancient nucleic acid.

iv. It's gene study is simple.

Ans2. a. Gammaproteiobacteria

B. Difference between mixed acid bacteria and butanediol bacteria-

1. Mixed acid bacteria- I. Produces more acids.

ii. It's drops the pH of the fermentor.

iii. Produces co2, H2 other than acids.

iv. These are gram negative bacteria.

butanediol bacteria- I. Produces less acetic acids.

ii. It's generates more neutral chemicals like acetyl methyl carbinol.

iii. The products are less inhibitory and maintain some what neutra pH of the biofermentor.

c. mixed acid fermentor - Lactic acid bacteria, E. Coli. They convert sugar to acids.

Butanediol fermentor- Enterobactor, Klebsiella