A. B. C. D. E. The TCA cycle will take a single turn to release the C14 molecule as carbon di oxide. The reaction involving isocitrate dehydrogenase to produce oxalosuccinate and alpha ketoglutarate from iso citrate produce the first carbon di oxide molecule.
F. No. Carbon di oxide can not be release at a different step.
Question: You buy a sample of glucose that has the radioactive carbon-14 (C) isotope at the...
OH cr nC-labeled glucose at C6 13C-labeled pyruvate at C3 a) When glucose is enriched with C at C6, it will form pyruvate enriched at C3 (above). The labeled Where does the label exist in succinyl-CoA after the 'C acetyl-CoA is processed in the first round of TCA (after a single action by the first four enzymes in TCA). Circle the atom/atoms where the label exists on the molecule below d pyruvate will be converted into 1'C acetyl-CoA and then...
Biochemistry: Answer the following questions about a molecule of glucose that has a radioactivr carbon at carbon 2. For each question, draw the molecule specified and indicate the location of the radioactive carbon. if there are multiple positions that the radioactive carbon may be located, include ALL positions in your answer. draw structure 2. Answer the following questions about a molecule of glucose that has a radioactive carbon (C14) at carbon 2. This molecule undergoes metabolism in a human cell...
3. Draw one turn of the citric acid cycle starting from a radio-labeled (*) acetyl-CoA shown below. Trace the fate of radio-labeled carbons and fully label each structure of intermediates through the citric acid cycle. Include the names of enzymes involved (in the table below), number each step, and indicate the names of each consumed or produced product. (Hint: There are symmetrical molecules present) (12 marks) CoA Нас 5. 2. 6 7. 3. 4. 8
You are carrying out an experiment in the lab to study the citric acid cycle. To do this, you add 14C-labeled pyruvate at carbon 3 (this is the methyl carbon) to metabolically active mitochondria. Based on your knowledge of the citric acid cycle, what is the location of the 14C-label in oxaloacetate after one turn of the cycle? How many turns of the cycle are required to release all of the14C-label as CO2? Be sure to justify your answers by...
4. (20 points) The reaction of pyruvate to acetyl-CoA and CO2 is the bridge reaction between glycolysis and the citric acid cycle. The carbon atoms in pyruvate are numbered and where they end up in the products are indicated CO0Θ 2 C=O + HS-CoA 3 CH3 Pyruvate S-COA 2C=O 3 CH3 Acetyl CoA Pyruvate dehydrogenase +1 CO2 a) Is C atom 1 in CO2 in a more reduced state or more oxidized state compared to its state in pyruvate? Why?...
13) When a molecule of glucose gets split during the energy investment phase of glycolysis, the products are two molecules of that then move to the energy payoff phase. A) Glyceraldehyde-3-phospahte B) Pyruvate C) Acetyl-COA D) NADH 14) Following glycolysis and the citric acid cycle, but before the electron transport chain and oxidative phosphorylation, the carbon skeleton of glucose has been broken down to CO2 with some net gain of ATP. Most of the energy from the original glucose molecule...
Draw the complete TCA cycle (Krebs or Citric acid cycle). Show the entry of acetyl- COA and the eight intermediates. Draw the chemical structures of each compound. Show the control point(s) in the pathway. Show where all CO2 is released and label the reactions where oxidation of carbon occurs.
Assume a cell has just completed glycolysis and the resulting pyruvate has a c-14 radio label on carbon -1. Highlight the carbon on the attached pages as it passes through the PDH complex and the TCA cycle until it is lost as CO2. If only a portion of the carbons are lost stop highlighting when the CO2 first starts to come off. ONLY ONE TIME THRU THE CYCLE ON EACH PAGE 2. Repeat question 1 but now assume the radio...
Which of the following is not a net product of the citric acid cycle for each molecule of pyruvate generated in glycolysis? Select one: a. 1 ATP (GTP) b. 1 acetyl coA c. 3 NADH d. 1 FADH2 Question 2 Which of the following is NOT an end product for an acetyl group that enters the citric acid cycle? Select one: a. ATP b. NADH c. FADH2 d. oxaloacetate Question 3 Once they have been processed through the citric acid...
Biochemistry question. Full answer and explanation would be appreciated. 4. Oxaloacetate uniformly labeled with "C (ie with equal amounts of "C in each of its carbon atoms) is condensed with unlabeled acetyl-CoA. After a single pass through the citric acid cycle back to oxaloacetate, what fraction of the original radioactivity will be found in the oxaloacetate? [10 points] 4. Oxaloacetate uniformly labeled with "C (ie with equal amounts of "C in each of its carbon atoms) is condensed with unlabeled...