Question

Question 20 4 pts You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain. 2. Digest the gold gene and a Ti plasmid with appropriate restriction enzymes. 3. Insert the gold gene into the Ti plasmid. 4. Amplify the gold gene with PCR. 5. Transfer the recombinant Ti plasmid into Agrobacterium tumefaciens. 6. Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome. 4,5, 2, 3, 1,6 4,2,5, 1, 6,3 4,2,3,5, 1,6 4,3,2,5, 1,6

Answer 1

first, amplify the gene using PCR, then clone the gene to the Ti plasmid, amplify the plasmid by transforming to bacteria, in the bacteria copy number of the plasmid increases, then transform Agrobacterium with the Ti plasmid, and infect the plants with the plasmid, then the plants with the plasmid can be selected using antibiotic.

so the order is 4,2,3,5,1,6

so the answer is c) 4,2,3,5,1,6.