Question

Name: ALCOHOL FERMENTATION EXPERIMENT Premise: Yeast are capable of carrying out an anaerobic energy production pathway called fermentation (anaerobic glycolysis). The waste products produced include CO2 and alcohol. All that is necessary to carry out this reaction is an adequate supply of sugar and a lack of oxygen. CO2 NADH + H TPP, NAD+ OH OH Mg2+ C=0 CH2 pyruvate alcohol CH3 decarboxylase CH 3 dehydrogenase CH3 Pyruvate Acetaldehyde Ethanol Without the potential to move into the tricarboxylic acid cycle the pyruvate that would move forward is converted to acetaldehyde by the enzyme pyruvate decarboxylase. The acetaldehyde is converted to ethanol by the enzyme alcohol dehydrogenase in a process that regenerates NAD+ to be used in glycolysis once again. Materials: - Fermentation bottle - Grape juice Universal indicator Lead indicator strip Digital balance Plastic spoon Air-lock Sucrose - Baker's yeast pH paper Weigh boat

Answer 1

2. Yeast is capable of carrying out an anaerobic energy production pathway through the anaerobic glycolysis. After completion of this pathway the waste products are co2 and alcohol. For successfully carrying out this pathway sucrose supply is necessary . Because sucrose is the main source of sugar, in yhe absence of O2.

3. Alcohol should be the main product. We will test it through the acetate strip, that indicate the ph of the solution. The ph of alcohol is 7.3 so, alcohol is neither acidic nor basic .

4. Must use the air-lock to avoiding oxidation. Yeast fermentation is a anaerobic process, if O2 is present then the reaction will prevented or stopped.