Question

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Lab Six: Fermentation Learning Objectives: • Explain the biochemistry of fermentation, substrates and products, conditions, and purpose for cells • Describe alcoholic fermentation of yeast, naming reactants and products Perform a pre-designed experiment to measure the rate of yeast fermentation of glucose under two different conditions. Propose hypotheses and make predictions based on them. Design and perform a novel experiment to test additional substrates for yeast fermentation using findings of the pre-designed experiment. Pre-Lab Worksheet Read the lab instructions before coming to the lab and answer the following questions: 1. Cellular Respiration takes place in the presence of and it involves two sub-cellular compartments: and 2. Fermentation takes place in the absence of cellular compartment, the and it involves one sub- 3. The metabolic pathway shared by cellular respiration and fermentation is 4. The cellular pathways that take place in the mitochondria are: The mechanism of ATP production in the mitochondria is known as and the product(s) is (are) 5. The substrate of yeast fermentation is and 6. In the lab, you will use a volume of fermentation (name of the simple apparatus) to measure the produced over a certain period of time as an indicator of yeast

Answer 1

1) cellular respiration occur in presence of oxygen and in two subcellular compartments cytoplasm and mitochondria ( kreb cycle take place ).

2) fermentation take place in the absence of oxygen and take place in cytoplasm .

3) common pathway between fermentation and cellular respiration is glycolysis . pyruvate converted to acetyl Co A and enter kreb cycle in cellular respiration whereas in fermentation pyruvate converted into ethanol.

4) cellular pathway that occur in mitochondria are cellular respiration , oxidative phosphorylation and electron transport chain. The mechanism of ATP production in mitochondrion is called oxidative phosphorylation.

5) yeast fermentation substrate is glucose (sugar) and products are ethanol and carbon dioxide.

6) Respirometer are used to measure the volume of CO₂produce during fermentation.