Question

Exercise 7: Aerobic Respiration and Alcohol Fermentation 57 JUUUUUUUUUUUUU the magnesium ions, and they are no longer available to act as cofactors. Without its cofactor, an enzyme changes its three-dimensional form, and, as a result, has the shape of its active site altered. This enzyme's normal substrate will not be able to fit into this altered active site, and thus, the enzymatic reaction has been noncompetitively inhibited. If glycolysis is inhibited by NaF, fewer pyruvic acid molecules will be produced; and, therefore, less Co, will be generated. Materials Yeast suspension Distilled water 0.02 M NaF 0.02 M MgSO, Fermentation tubes Procedure 1. A fresh yeast suspension of one package of yeast in 400 mL of 2% glucose solution will be provided. 2. Clean fermentation tubes, 1 through 5. (Make sure that all five fermentation tubes are of the same size.) 3. Shake up the yeast-glucose suspension and place 25 ml of the mixture in each fermentation tube. 4. Then add the following to each tube: No. 1: 10 ml distilled H,O No. 2: 5 mL 0.02 M NaF and 5 mL distilled H,O No. 3: 5 mL 0.2 M NaF and 5 mL distilled H.O No. 4: 5 mL 0.02 M MgSO, and 5 mL distilled H.O No. 5: 5 mL 0.2 M MgSO, and 5 mL distilled H.O NOTE: Use the graduate cylinder marked NaF only with the Nal and the graduate cylinder marked MgSO, only with MgSO 5. Tip the tubes to remove air from the upright arm. 6. Incubate at 37° C for 1 hour. 7. Record the amount of gas produced by measuring the displacement of fluid in the upright arm of each tube: No. 1: _ No. 2: No. 3: _ No. 4: _ No. 5: 8. Explain your results in light of the information provided in the introduction to this experiment. REVIEW QUESTIONS 1. What is the start compound for both aerobic respiration and fermentation 2. What gas does the germinating soy beans take up? 3. What gas causes the bubble to get larger during the fermentation procedure? 4. Why don't the dry, nongerminating soy beans do not take up oxygen gas? 5. What was the function of KOH in the aerobic respiration procedure? 6. If you performed the aerobic respiration procedure but did not soak the cotton balls in KOH, hypothesize on how the results might have been different.

Answer 1

1. Glucose or any other sugar

2. Carbondioxide

3. Carbondioxide

4. Dry non germinating soyabean does not take Oxygen gas because they will not show any metabolic activity in the absence of water.

5. Potassium Hydroxide is used in the experiment of aerobic respiration so that it can absorb carbon dioxide. Carbon dioxide is generated in aerobic respiration during pyruvate oxidation and tricarboxylic acid cycle.

6. This will lead to accumulation of carbon dioxide. This may result in carbon dioxide toxicity inside the test tube and the seeds may not germinate.

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