Upon removal of bacteriophage coats from infected bacterial cells, where was the label for the DNA?
The labelled DNA was found in the genome of the bacteria.
Messelson and Stahl experiment :
Radiolabelled phosphorus present in protein coat of the bacteriophage + E coli cells = radioactivity was found in phage ghost (protein coat of bacteriophage) = protein is not transferred from virus to bacteria, so it is not a genetic material
Radiolabelled sulphur present in genome of the bacteriophage + E coli cells = radioactivity was found in the genome of E coli = DNA was transferred from virus to bacteria so it is the genetic material
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Upon removal of bacteriophage coats from infected bacterial cells, where was the label for the DNA?
QUESTION 22 Upon removal of bacteriophage coats from infected bacterial cells, where was the label for the DNA? A. in the cell pellet B. in the supernatant C. in bacteriophage coats D. in both the cell pellet and supernatant
If 100% of the DNA contents of a transducing bacteriophage were DNA from the host chromosome, what would happen when the bacteriophage infected a new bacterial cell? Group of answer choices The bacteriophage would be converted from a lysogenic phage to a lytic bacteriophage. The new bacterial cell would die. No more bacteriophage would be synthesized, as there is no bacteriophage genome available to direct synthesis. More bacteriophage would be synthesized, resulting in death of the new bacterial cell. The...
Suppose you label bacteriophage T2 with 15N for 10 rounds of bacterial infection 15N will label DNA (i.e., you use 15 N instead of P^32) and not protein. a) What percentage of the viral DNA strands will be labeled with 15 N? b) Now, after labeling bacteriophage T2 for 10 rounds of infection with l5N, your lab partner forgot to include 15N in the growth media for an unknown number of rounds of replication. You take out a sample and...
The Hershey Chase experiment using radioactively labeled bacteriophage to infect bacteria proved that DNA was the genetic material. If you were reviewing those experimental results, which of the following would provide evidence that DNA was the genetic material? Select all that would apply. a) The DNA was localized in the cytoplasm of the bacterial cell, which is the same site that the bacterial chromosome is located. b) Radioactively labeled viral proteins were only found outside the cell and the viral...
What role does a bacteriophage play in transferring DNA from one bacterial cell to another?
When a bacteriophage carrying bacterial DNA infects a new bacterium, Multiple Choice the recipient is usually killed. the recipient keeps the transferred DNA in storage but does not replicate it. O o oo ) it transfers bacterial DNA from the donor bacterium to the recipient bacterium. only viral DNA is transferred to the recipient bacterium.
The bacteriophage used in this experiment reproduces by the lytic cycle. Suppose a similar experiment were conducted with a bacteriophage that uses the lysogenic cycle. How would the effect on the infected bacteria MOST likely differ? A. The bacteriophage would attach to the bacteria and reproduce in the extracellular space. B. The bacteriophage would become permanently dormant and unresponsive to environmental cues for reproduction. C. The bacteriophage would attach its genes to the bacteria’s DNA, allowing its genes to be...
multiple answers are possible! Which of the following statement about DNA is FALSE? When bacteriophage DNA was shown to be the agent necessary for production of new phages, this was the early experiment demonstrating that DNA is the hereditary material. During DNA replication, two replication forks are formed to complete the synthesis of the entire chromosome in eukaryotes. During bacterial DNA replication process, replication forks move in opposite directions away from the origin of replication The molecular structure of DNA...
ΦC31 is a type of bacteriophage that infects Streptomyces bacteria. One gene in the bacteriophage genome specifies a recombinase called ΦC31 integrase that works through a mechanism slightly different from that of the recombinase shown in Fig. 6.30. Most importantly, the two target DNA sequences are different from each other. One called attP is 39 base pairs and is found on the circular bacteriophage chromosome, while the other—attB—is 34 base pairs long and is located on the much larger circular...
Label the image to review major targets of drugs acting on bacterial cells. Cephalosporins Protein synthesis inhibitors acting on ribosomes Cell membrane Sulfonamides (sulfa drugs) Cell wall inhibitors Penicillins Polymyxins Erythromycin Aminoglycosides Quinolones Folic acid synthesis DNA/RNA Rifampin