True or False Measurements of gene expression at the transcriptional level typically require making cDNA from...
Real time PCR very accurately quantifies the amount of template DNA because it detects before any reagents become limiting Tor F Measurements of gene expression at the transcriptional level typically require making cDNA from mRNA Tor F. An important distinction between traditional Sanger (dideoxy) DNA sequencing and 2nd generation (NGS) DNA sequencing is that NGS gives longer DNA sequence "reads" Tor F.
Which does not affect gene expression at the post-transcriptional level? Choose one: O A. DNA methylation O B. small interfering RNAs O C. microRNAs O D. nucleotide sequences in the untranslated regions of an mRNA
8. Control of gene expression in eukaryotic cells occurs at which level(s)? A. only the transcriptional level B. epigenetic and transcriptional levels C. epigenetic, transcriptional, and translational levels
TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION CAN INVOLVE A. BINDING OF HORMONE-RECEPTOR COMPLEXES TO ENHANCERS B. REPRESSOR-MRNA BINDING C. EXON MUTATIONS D. ALTERNATIVE POLYADENYLATION AND SPLICING PATHWAYS
Which of the following is not true regarding regulation of gene expression in eukaryotes? In eukaryotic cells, transcriptional activators often work by recruiting the transcriptional macinery to gene promoters DNA methylation is an example of an epigenetic mark that can be inherited Acetylation of histones can result in a more open chromatin structure to allow transcription to occur Different genes can be turned off or on between different organs or stages of development Transcription factor DNA sequences are bound by...
What transcriptional regulation mechanism unique to mammals allows for selective gene expression from one allele only? Explain how this impacts expression of IGF2.
Q1)Which of the following is not involved in control of gene expression in eukaryotes? A. Change to DNA sequence. B. Alternative splicing. C. Export of mRNA from the nucleus. D. Transcriptional repression. E. Destruction of mRNA Q2) Trans-acting regulators of gene expression include? A. promoters. B. histone deacetylases C. introns D. silencers E. the TATA box. Q3) Gene regulation in eukaryotes often involves which of the following, which are not also used by prokaryotes? A.RNA polymerase B.Transcription factors C.Histone modification...
Tissue-specific microarray analysis facilitates the comparison of gene expression in different tissues. In order to prepare a sample for microarray analysis, messenger RNA (mRNA) is extracted from a particular tissue, reverse transcribed to generate complementary DNA (cDNA), and labeled with a fluorescent probe. The microarray below shows comparison of gene expression in stomach and intestinal tissues. The cDNA from stomach tissue was labeled with red fluorescent probes, whereas the cDNA from the intestinal tissue was labeled with green fluorescent probes....
Which of the following is the most appropriate indication of gene expression level? A ONA 6. Protein CRNA DmRNA QUESTION 14 In reality, what is being directly quantified in real-time rt-PCR to estimate the gene expression level? OM Protein levels by amplifying with ribosomes using fluorescently tagged amino acids B. DNA which is amplified with gene-specific primers and measured for fluorescence Reverse transcriptase levels which indicates rate of mRNA transcription O mRNA which is amplified with gene-specific primers and measured...
4. (2pts) Which of the following is true concerning expression levels of the BIS gene in cells expressing miR-5? BIS gene expression is increased in these cells BIS gene expression is decreased in these cells BIS gene expression remains unchanged in cells expressing miR-5 Transcription initiation of BIS is negatively regulated Question 4 2 pts 5.(2pts) Which of the following are true of p101 expression in these cells? o increased amounts of the p101 protein due to low levels of...