Homework Answers
mRNA is the most appropriate indication of gene expression level.
mRNA which is amplified with gene specific primers and measured for florescence is being directly quantified in real time rt-PCR to estimate the gene expression level.
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Which of the following is the most appropriate indication of gene expression level? A ONA 6....
4. (2pts) Which of the following is true concerning expression levels of the BIS gene in...
4. (2pts) Which of the following is true concerning expression levels of the BIS gene in cells expressing miR-5? BIS gene expression is increased in these cells BIS gene expression is decreased in these cells BIS gene expression remains unchanged in cells expressing miR-5 Transcription initiation of BIS is negatively regulated Question 4 2 pts 5.(2pts) Which of the following are true of p101 expression in these cells? o increased amounts of the p101 protein due to low levels of...
9. Analyze the diagram below of a nuclear response for gene expression: Which of the following...
9. Analyze the diagram below of a nuclear response for gene
expression:
Which of the following is this cell using to control all of the
other events to regulate gene expression?
a. phosphate bonded to a transcription factor
b. phosphorylation cascade with multiple steps
c. specific hormone that initiates a response
d. transcription housed outside of the nucleus
10. Below is a model of a signal transduction pathway that results
in the transcribing of mRNA:
Referencing the model, what...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The test requires a lab to run the PCR Collateral RNAse activity is activated when RNA guide finds the target RNA (COVID19). If the Reporter RNA is cleaved by CAS13 it binds to the test strip, 2 lines will appear test positive The Cas13 protein attached to the RNA guide has collateral RNA activity This test does not require a PCR step Question 2 2...
When the expression level of the lac operon is quantitated, the amounts of the gene products...
When the expression level of the lac operon is quantitated, the amounts of the gene products of the loc z. lacy and loc a genes are present in the ratios 10:52 respectively. ONE of the 5 following statements regarding this is FALSE. Identify the FALSE statement. O Shine-Dalgarno sequences can vary in their effectiveness O All proteins are not required in equal amounts by a cell O Different promoter strengths can produce different amounts of mRNA O Secondary structures may...
Answer the following questions that do not have an answer. Creating a graph is not necessary....
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
after the pGlo vector was...
QUESTION 4 Which of the following is NOT a mechanisms of protein expression? Specific transcription factors...
QUESTION 4 Which of the following is NOT a mechanisms of protein expression? Specific transcription factors binding to enhancers Export of mRNA into the cytoplasm DNA polymerase phosphorylation 5'UTR secondary structure blocking AUG QUESTION 5 Suppose you have all the necessary proteins for transcription of a gene present AND active/turned on AND in the right location within the cell. Under what conditions would you NOT see transcription of that gene? UmRNA has already been transcribed from that gene but has...
QUESTION 6 1 points Save Answer Which point of gene expression will cause the most rapid...
QUESTION 6 1 points Save Answer Which point of gene expression will cause the most rapid change in protein that controls a particular trait? O transcription O Translation O post-translational modifacation O chromatin remodeling
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
Which technique would you use if you wanted to < Identify all the regions of open...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
need to know correct answers 26. B. Which of the following enzymes is associated with the...
need to know correct answers
26. B. Which of the following enzymes is associated with the repression of transcription? methyl transferase DNA polymerasex acetyl transferase histone kinase none of the above Section 2 - True/False (2 points each) 27. TFIIB regulates tissue or cell type-specific transcription 28._1 Eukaryotes have three different RNA polymerases that each can transcribe non-protein coding genes. 29. T The P site of the ribosome refers to peptidyl. be For T heoph UND 30._F_The input genetic material...
4. (2pts) Which of the following is true concerning expression levels of the BIS gene in cells expressing miR-5? BIS gene expression is increased in these cells BIS gene expression is decreased in these cells BIS gene expression remains unchanged in cells expressing miR-5 Transcription initiation of BIS is negatively regulated Question 4 2 pts 5.(2pts) Which of the following are true of p101 expression in these cells? o increased amounts of the p101 protein due to low levels of...
9. Analyze the diagram below of a nuclear response for gene
expression:
Which of the following is this cell using to control all of the
other events to regulate gene expression?
a. phosphate bonded to a transcription factor
b. phosphorylation cascade with multiple steps
c. specific hormone that initiates a response
d. transcription housed outside of the nucleus
10. Below is a model of a signal transduction pathway that results
in the transcribing of mRNA:
Referencing the model, what...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The test requires a lab to run the PCR Collateral RNAse activity is activated when RNA guide finds the target RNA (COVID19). If the Reporter RNA is cleaved by CAS13 it binds to the test strip, 2 lines will appear test positive The Cas13 protein attached to the RNA guide has collateral RNA activity This test does not require a PCR step Question 2 2...
When the expression level of the lac operon is quantitated, the amounts of the gene products of the loc z. lacy and loc a genes are present in the ratios 10:52 respectively. ONE of the 5 following statements regarding this is FALSE. Identify the FALSE statement. O Shine-Dalgarno sequences can vary in their effectiveness O All proteins are not required in equal amounts by a cell O Different promoter strengths can produce different amounts of mRNA O Secondary structures may...
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
after the pGlo vector was...
QUESTION 4 Which of the following is NOT a mechanisms of protein expression? Specific transcription factors binding to enhancers Export of mRNA into the cytoplasm DNA polymerase phosphorylation 5'UTR secondary structure blocking AUG QUESTION 5 Suppose you have all the necessary proteins for transcription of a gene present AND active/turned on AND in the right location within the cell. Under what conditions would you NOT see transcription of that gene? UmRNA has already been transcribed from that gene but has...
QUESTION 6 1 points Save Answer Which point of gene expression will cause the most rapid change in protein that controls a particular trait? O transcription O Translation O post-translational modifacation O chromatin remodeling
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
need to know correct answers
26. B. Which of the following enzymes is associated with the repression of transcription? methyl transferase DNA polymerasex acetyl transferase histone kinase none of the above Section 2 - True/False (2 points each) 27. TFIIB regulates tissue or cell type-specific transcription 28._1 Eukaryotes have three different RNA polymerases that each can transcribe non-protein coding genes. 29. T The P site of the ribosome refers to peptidyl. be For T heoph UND 30._F_The input genetic material...
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