Question

Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The test requires a lab to run the PCR Collateral RNAse activity is activated when RNA guide finds the target RNA (COVID19). If the Reporter RNA is cleaved by CAS13 it binds to the test strip, 2 lines will appear test positive The Cas13 protein attached to the RNA guide has collateral RNA activity This test does not require a PCR step Question 2 2 pts What is the difference between regular PCR and RT-PCR? In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In PCR, the temperatures vary more than in RT-PCR which can work at the same temperature throughout the entire process. In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified.

Answer 1

Questions 1. Ans - c , if tha reporter RNA is cleaved byCAS13 it binds to the test strip, 2 lines will appear= test positive.

Questions 2. Ans d