Question

D Question 3 2 pts When and how does the RT-PCR test detect the presence of the virus? After PCR, by adding a quencher dye which is activated when the viral DNA is available in high concentrations During PCR by adding fluorescent primers that are detected through a special laser After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. D Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR is less expensive than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken

Answer 1

RT PCR stand for real time pcr. It is also called Q PCR. It's uniqueness lies in the fact that it tells us the data in the real time scenario. It is a widely used molecular biology technique which has been used to study gene expression. Studying mechanism of virus is also a task of it. Q PCR contains a probe which is aligned in such a way that the fluorescent reporter is bound in place by the quencher. However post denaturation when annealing occurs, the probe annealed itself to the virus genetic material and expresses the fluorescent reporter which shows fluorescence and thus gets detected.
Answer-After PCR, by adding fluorescent dye which will be activated once the sample goes through laser.

CRISPR is a relatively new technology in the field of biotechnology. It is used to edit the molecular arrangement of a gene by altering and modifying it. Cas9 acts a scissor in this method. Therefore it is much more reliable and accurate than RT PCR

Answer-CRISPR is more accurate than RT PCR