Question

What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR which can work at the same temperature throughout the entire process.

Answer 1

The answer for the question is that in RT-PCR, you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence in amplified.

This makes it easier for tracking the amplification in real time. Whereas in regular PCR, the end products have to be analysed by techniques like agarose gel electrophoresis in order to ensure that the amplification has taken place.