Question

Question 10 0.5 pts Order the steps in RT-PCR: • Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse transcriptase), dNTPs, and a buffer with Mg2+ Incubate the reaction appropriately. • Degrade the RNA strand with base, leaving just the cDNA. • cDNA is produced, which is single-stranded and complementary to the RNA of interest. • Double-stranded DNA for the region of interest is produced. • Add two primers (one forward and one reverse) located within the sequence of the RNA of interest. Also add DNA polymerase, dNTPs, and a buffer with Mg2+. Cycle temperatures appropriately. • Obtain an RNA sample. Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse transcriptase), dNTPs, and a buffer with Mg2+. Incubate the reaction appropriately. Degrade the RNA strand with base, leaving just the cDNA. [Choose] cDNA is produced, which is single-stranded and complementary to the RNA of interest. [Choose ] Double-stranded DNA for the region of interest is produced. [Choose] [Choose] Add two primers (one forward and one reverse) located within the sequence of the RNA of interest. Also add DNA polymerase, dNTPs, and a buffer with Mg2+. Cycle temperatures appropriately. Obtain an RNA sample. [Choose]

Answer 1

Answer

Step 1: Obtain a RNA sample

Step 2: Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse trasnscriptase) , dNTPs, and a buffer with Mg2+. Incubate the reaction appropriately.

Step 3: Degrade the RNA with base leaving just the cDNA

Step 4: cDNA is produced which is single stranded and complementary to the RNA of interest.

Step 5: Double stranded DNA for the region of interest is produced.

Step 6: Add two primers (forward and reverse) located within the sequence of the RNA of interest. Also add DNA polymerase, dNTPs, and a buffer with Mg2+. cycle temperature appropriately.