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You want to diagnose if a patient has tuberculosis. A sputum sample (a respiratory tract sample...

You want to diagnose if a patient has tuberculosis. A sputum sample (a respiratory tract sample taken when a person coughs) is given to you. Based on the staining procedures you conducted in lab during week 2, name the staining procedure you would conduct, the dyes used, and describe what you would see on the slide you prepare. Why is this considered a differential procedure?

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Staining procedure used is Acid Fast Bacillus staining.AFB staining.

We use ZIEHL NEELSON method of afb staining.

Primary dye is carbol fuchsin and counter stain is methylene blue or malachite green.

We see Acid fast bacilli as red cells. Where background will be stained blue or green based on counter stain.

This is differential staining because only acid fast bacilli retain carbol fuchsin stain when washed with acid alcohol. Other cells destain when washed and take counter stain. That is principle of differential staining.

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