Question

please answer me question ( a) and (b) in no more than 6 sentences. thanks.

How could you design a drug to target nuclear import that could prevent HIV infection? How might your design impact the normal function of cells, leading to side effects?

Answer 1

Answer - la In order for the HIV protan to be imported the nuleus, the protein should have nulas localization Eignals (NLS) that can be recognized by importing. The pre- initiation Complen of HV Contains bour Karyophillic proteins mA, IN, vpr (viral digin) and LEDGE/ p 75 Chost cell sigin to J IN is the protein that can be targeted to prevent mulear import. in interaits with importin alpha in the nucleus and can be targeted. It has an NLS in 161-173 amino auid lolid d method to prevent the interaction b/w IN and importin is use of cyclic and linear peptides. The peptides can interact with the NLS domain of a Karyophillic protun. - As a result, the domain is masked and the importin connot bind. Conversely the peptides minic the domain and compete with the in for interaction with importin. similarly, small molemt inhibitors. such as Styrylquinolines can block in linked HW replication by inhibiting nuclear import.

b) Importin alpha " used for nulear transport of other protuns. These drugs can only inhibit nuclear import. However, it the viral DNA s already in the nucleus, Then HIV can still replicate at the peptide mimiy the domain, then. limportin will bind to this pertide. The Si co n tence nuelal Export of other Essential cellular prot uns will be also be intübited. This will afbut the target cell drastically causing cell deapth botones bet a better approach will be to masha the domain by Louding of the Nes of AN. The Effect of in inhibitors is seen at high concentration which may affect the host cell negatively too due to drug tonicity Sut please rate my Answich