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Describe the purpose of the polymerase chain reaction, along with the risks associated with the increase in target DNA strand length.
Ans.
PCR is a laboratory technique which involves sythesis of millions of copies of DNA from a single strand of DNA.
This technique, in essence is an amplification method, whereby the little amount of DNA present in tissue sample is copied in amount sufficient for the analysis.
This method got it's name from the key component of the reaction, "DNA polymerase" which carry out the amplification.
PCR is used very widely in the analysis of clinical samples for the presence of infectious agents like viruses, bacterias ... etc.
An other emerging area of PCR use is mutation detection which is implicited in may diseases e.g., sickle cell anemia and cystic fibrosis.
In case of target DNA strand length is increased from that optimal for the intended reaction, than it may show false positive or false negative results.
Also there may be a formation of hairpin like structure because of the annealing of the DNA strands with each other resulting in false results.
Note: Please provide a professional 4th-year level with a clear explanation or LEAVE the question for...
Note: Please provide a professional 4th-year level with a clear explanation or LEAVE the question for someone else to answer! Q- Discuss a potential greatest source of nanoscale materials that may be inadvertently digested by the human body, as well as the means by which these materials enter the body.
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Which of the following events would not improve (increase) a company's return on equity? (Assume all else remains the same.) a. Retirement of long-term debt with cash b. Sale of common stock for cash c. Reduction in operating expenses Select one: a O b bandc a and c a and b C с None of the events would improve a company's return on equity.
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Calculate the magnetic field due to (i) a very thin current sheet in the xy-plane (infinitely sized in x, y) with a...
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Numerically verify that the Central Limit Theorem is valid even
when sampling from non-normal distributions. Suppose that a
component has a probability of...
Can someone write me a clear understanding explanation to the following question? Thank you. Please state why you think you have the correct answer. Example 1.13: Which of the following compounds undergoes a nucleophilic substitution reaction with ethyl chloride at the greatest rate. A. H3CCHClNH2 B. ClH2CCH2NH2
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Question 5 0/3 pts If the interest rate on a note is 12.5% and the principal was $57,000, what is the maturity value of the note, if the term of the note is 5 months? (Round your final answer to the nearest dollar.) You Answered $64,125 Correct Answer $59,969 O $61,156 $57,000
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Two kilograms of water execute a Carnot power cycle. During the
isothermal expansion, the water is heated until it is a saturated
vapor from an initial state where the...
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1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
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QUESTION 1 All organisms must duplicate their DNA with extraordinary accuracy before each cell division A mixture of proteins accompishes DNA replication. Each of the proteins act independent of one another yet held together in a large multi-enryme complex that moves rapidly along the DNA. Imagine a tiny sewing machine composed of protein parts and powered by nucleosidetriphosphate hydrolases Match the protein subunit or factor of the replication machine to the...