Question

Give all 14 possible answers with drawing

In the schematic, the position of a two turn RSS (dark triangle) is shown. Below the schematic, I provide a key to various DNA sequence motifs (labeled A-E). In the answer box, indicate the appropriate DNA motif (using the key) for each 5' and 3' location that would immediately flank (contact) each of the 7 gene segments that is required for VDJ re-arrangment. This means that you will have a total of 14 answers and that answers can be used more than once. Assume that all gene segments are oriented in the same direction. A = 2 turn RSS B = Switch Region C = no RSS D = Both 1 turn and 2 turn RSS E = 1 turn RSS

explain this there should be 14 outcomes

Answer 1

This question is answered based on the following VDJ recombination rules:

1. RSS region is located at the 3' side of V segment

2. 5' side of J segment

3. Both sides of D segment4. VDJ recombination follows a 12/23 rule (one turn rss will join only with two turns

rss)

Please see the attached image for the 14 possible recombinations.

| CE A AE | B Possible Recombinations: | | 一口一口一口一口一回 E- - V-D- | 2,一口一回一回—— —TIATE —— TEA AE | - Vi - D-2 3 一口一回 ————国口回国一 AE E A - VI-D - - 4 回——zg回一回一 AE + E -Vi - D- T = 50一四一回一回一回 E- c AE E- |-VI - D1-12 一 回回回回 - -VI-D - - 1 口— ——— —— — —— — A E A g -V-by-72- | 4 B HB CAE A

* ро- [pi-s- EA A В — — 4р ( т — V2 - - J, - - - | |Б| - V2 -D-J, - i-n-gol-CI- С Е А А Е A E E A B — V2 -2 -1, - 1-о-о- р — 1 — 2 ——- А Е А - Е В — V, -р, -, — 12 с-м | | 43-р-р-р-18- - V2 - D-J, — 13. -Nj]— 4| ) ап рН 8— - V2 - - - с A E E A 13 с AE AE 14 с E A E - V2 - D -Т, -