1.) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon.
2.) When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout?
3.) Many DNA-binding proteins that affect gene expression are allosteric. What is allostery and why is it such a common mechanism of gene regulation?
IPTG IS a inducer of lac operon which binds with the repressor of lac operon as like allolactose and releases the repressor from operator and synthesizes all the mRNA of lactose METABOLISM.
They use them to study the sequences near the promoter and to explore the role of this part.thank you
1.) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
I really need help with these questions!! 2. Bernard Davis tested the "cross-feeding" interpretation of some data that showed the phenotype of one microbe as capable of being changed by another microbe. His contribution can be summarized as: A) using one strain of microbes as food for another, and then evaluating the impact upon microbial phenotype. B) combining microbes with complementing auxotrophs in a single tube and then analyzing the cells of gene exchange. C) placing a barrier between bacterial...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...