Question

Does COVID 19 have

a 5’ CAP and a  Poly(A) tail? Site your sources,

Answer 1

Answer:

Yes, COVID 19 (Which is the name of the disease) and it is caused by the virus name: SARS-COV-2.

It does have 5’- methylated cap and 3’- polyA tail along with UTRs in its mRNA.

Note:

Since links of websites can't be directly pasted as per answering guidelines so the source website screenshots are given below (website link can be seen in the image):

Source 1: (This site would provide more resear paper sources stating that COVID-19 has 5’- methylated cap and 3’- polyA tail along with UTRs)

+ − c geneticeducation.co.in/novel-coronavirus-covid-19-structure-genome-and-testing/ mu promovem queue the GenBank. Some of the common genome features of the coronavirus strains are explained here: 1. The ORF1a and 1b are the largest genes of coronavirus spanning two-third of the entire genome. It majorly encodes non-structural proteins which help in replication and transcription in the host. 2. All viral mRNA has 5'- methylated cap and 3'- polyA tail with UTRs. 3. The untranslated region of 5' end is up to 528 nucleotides long while the 3’UTR region of the gene is 288 to 506 nucleotides long. 4. The 3' UTR region possesses duplicated octameric GAGA GAGA sequence upstream to polyA tail. 5. All the ORFs start with the same AUG codon. 6. All the structural proteins (S, E, M and N) are transcribed in 5' to 3' direction by RNA dependent RNA polymerase (RdRp). Structure:

Source 2:

• Journal Name: Journal of Medical Virology.
• Paper name: Emerging coronaviruses: Genome structure, replication, and pathogenesis
• Authors: Yu Chen, Qianyun Liu, Deyin Guo
• First published: 22 January 2020

+ + C onlinelibrary.wiley.com/doi/full/10.1002/jmv.25681 Wiley Online Library Access by WILEY. Search Search Q Login / Register Login / Register DE SECTIONS PDF Y TOOLS SHARE Figures References Related Information effective therapies and vaccines against COVs. 2 CORONAVIRAL GENOME STRUCTURE AND REPLICATION Metrics Citations: 33 Am score 179 Details © 2020 Wiley Periodicals, Inc. COVs belong to the subfamily Coronavirinae in the family of Coronaviridae of the order Nidovirales, and this subfamily includes four genera: Alphacoronavirus, Betacoronavirus, Gammacoronavirus, and Deltacoronavirus (Figure 1A). The genome of CoVs is a single- stranded positive-sense RNA (+SSRNA) (~30 kb) with 5'-cap structure and 3-poly-A tail. The genomic RNA is used as template to directly translate polyprotein 1a/1ab (pp1a/pp1ab), which encodes nonstructural proteins (nsps) to form the replication-transcription complex (RTC) in a double-membrane vesicles (DMVs).6 Subsequently, a nested set of subgenomic RNAs (SgRNAs) are synthesized by RTC in a manner of discontinuous transcription.7 These subgenomic messenger RNAs (mRNAs) possess common 5'-leader and 3'-terminal sequences. Transcription termination and subsequent acquisition of a leader RNA occurs at transcription regulatory sequences, located between open reading frames (ORFs). These minus-strand sgRNAs serve as the templates for the production of subgenomic mRNAs.8, 9 Check for updates Keywords coronavirus epidemiology pathogenesis respiratory tract virus classification zoonoses Identity 54% Identity 58% Identity 43% HCOV 229E HCOV-NL63 a-Cols TGEV Bat SL ZC45 Bat-SL ZXC21 2019-nCov Funding Information ppia opib a-Cols • China National Science and Technology Ba|36 EM N -HCOV 229€