Question

Question 4) Trans-splicing is a special form of RNA splicing that occurs in 70-80% of all genes in the soil nematode C. elegans. In trans-splicing, the exons from two different primary RNA transcripts produced in different portions of the genome are joined end-to-end and ligated. Do you consider these trans-spliced sequences of real genes or are they instead the product of a fusion of two genes? Question 5) Recently researchers discovered a new class of mRNA regulators named miRNAs. miRNAs are small - 21 nucleotide long, non-coding RNAs that target mRNAs and repress their expression into protein, miRNAs can dramatically reprogram the expression of many genes into protein and influence phenotypes. If you consider genes protein coding sequences, how would you categorize miRNAs?

Answer 1

Answer(4): Trans-splicing is a special form of RNA processing where exons from two different primary RNA transcripts are joined end to end and ligated.

So trans-spliced RNA sequences are the product of a fusion of two genes.

Answer(5): miRNAs are not generally located within coding exons,miRNAs are small non-coding RNAs.

Most miRNAs are transcribed from DNA sequences into primary miRNAs (pri-miRNAs) and processed into precursor miRNAs (pre-miRNAs) and mature miRNAs.

So we can categorize miRNA as:

• Primary miRNA
• Precursure miRNA
• Mature miRNA