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1. There are three basic steps in DNA extraction - A. breaking open the cells, B....
Name Date 5) What is the first step in the DNA extraction process? 6) What cells...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
1. Blend cells to DNA?) Keep in and release the - (Which organelle holds the solution...
1. Blend cells to DNA?) Keep in and release the - (Which organelle holds the solution to maintain water balance (osmotic balance). the mixture to pellet nuclei. the buffer solution, including the rest of the cell contents other than nuclei. 4. Resuspend the pellet of nuclei with 5. Check your progress: add a drop of stain to your suspension to stain and see the tiny nuclei under your microscopes. 6. Add EDTA to _the nuclear membranes. 7. Add SDS, a...
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic...
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic building block of a nucleic acid (DNA or RNA) is called a and consists of three parts. The three parts are: A. B. C. 2. Describe TWO basic differences between the structure of DNA and the structure of RNA: A. B. 3. Describe the basic difference between the function of DNA and RNA: 4. Name (write out) the names of the FOUR bases of...
c) The steps or rungs of the DNA ladder are composed of phosphate group 4 Deoxyribose...
c) The steps or rungs of the DNA ladder are composed of phosphate group 4 Deoxyribose 15. Use Figure 2 and 3 of the lab to compare the genome of a human with a mouse, fruit fly and yeast. paired in a specific way. d) Adenine in one DNA strand always pain with thymine ) Bases in opposite strands of a DNA molecule are linked together by hydrogen in the other strand and bonds. Yeast Human Mouse Fruit Fly Number...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
Choose the ONE best answer 1. For solution/sample storage, you must label all containers with A....
Choose the ONE best answer 1. For solution/sample storage, you must label all containers with A. Name of the chemicals 2. You used Fume Hood to: A. Handle chloroform B. Homogenize C.Fiter using cheesecloth D.A 3. The least resistant type of organism/ pathogen include: A. Enveloped virus B. Bacteria C. Fungi D. Spore 4. The most resistant type of organism/ pathogen include: A. Enveloped virus B. Bacteria C. Fungi D.Spore 5. For the disposal of hazardous chemicals you will use...
I need help answering the following microbiology questions. 1. The three tubes in the image to...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
1. A calorie is a measure of the energy content of food. a. True b. False...
1. A calorie is a measure of the energy content of food. a. True b. False 2. Which chemical in the alcohol metabolic pathway is most toxic? a. ethanol b. acetaldehyde c. acetic acid d. none are toxic e. all are equally toxic 3. Which of the following are risks of long term regular use of alcohol. a. Increased cancer rates. b. Infertility and impotence. c. Diabetes. d. Liver disease. e. All of the above. 4. An amino acid is...
Construct a flow chart describing the seperation of the mixture and the isolation of each compound...
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
1. Blend cells to DNA?) Keep in and release the - (Which organelle holds the solution to maintain water balance (osmotic balance). the mixture to pellet nuclei. the buffer solution, including the rest of the cell contents other than nuclei. 4. Resuspend the pellet of nuclei with 5. Check your progress: add a drop of stain to your suspension to stain and see the tiny nuclei under your microscopes. 6. Add EDTA to _the nuclear membranes. 7. Add SDS, a...
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic building block of a nucleic acid (DNA or RNA) is called a and consists of three parts. The three parts are: A. B. C. 2. Describe TWO basic differences between the structure of DNA and the structure of RNA: A. B. 3. Describe the basic difference between the function of DNA and RNA: 4. Name (write out) the names of the FOUR bases of...
c) The steps or rungs of the DNA ladder are composed of phosphate group 4 Deoxyribose 15. Use Figure 2 and 3 of the lab to compare the genome of a human with a mouse, fruit fly and yeast. paired in a specific way. d) Adenine in one DNA strand always pain with thymine ) Bases in opposite strands of a DNA molecule are linked together by hydrogen in the other strand and bonds. Yeast Human Mouse Fruit Fly Number...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
Choose the ONE best answer 1. For solution/sample storage, you must label all containers with A. Name of the chemicals 2. You used Fume Hood to: A. Handle chloroform B. Homogenize C.Fiter using cheesecloth D.A 3. The least resistant type of organism/ pathogen include: A. Enveloped virus B. Bacteria C. Fungi D. Spore 4. The most resistant type of organism/ pathogen include: A. Enveloped virus B. Bacteria C. Fungi D.Spore 5. For the disposal of hazardous chemicals you will use...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
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