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Name Date 5) What is the first step in the DNA extraction process? 6) What cells...
During DNA extraction . The lysis solution provides a detergent and an enzyme. What is the...
During DNA extraction . The lysis solution provides a detergent and an enzyme. What is the enzyme included, and what is its purpose? What is a specific molecule that this enzyme acts upon and what is this molecule’s connection to DNA? Why is a detergent important in breaking open cells
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1....
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
Based on solubility, density, and polarity information what will happen if a caffeine extraction and sublimation...
Based on solubility, density, and polarity information what will happen if a caffeine extraction and sublimation experiment is carried out at 100°C? (Note: show the outline, the experiment might not work perfectly in the actual lab, but a hypothetical outline is needed). Procedure below: Boil 20mL DI water. Place 1st tea bag in beaker taken off heat and cool to handling temperature. Squeeze tea from bag without breaking it. Boil the mixture. Repeat steps with 2nd teabag. Cool to room...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
look over the expeirment instructions in photos 1-3 then answer questions 1-4 in the last photo...
look over the expeirment instructions in photos 1-3 then
answer questions 1-4 in the last photo
Exercise 1 Extraction of DNA In this exercise, you will extract and observe DNA from split peas. Note: The night before beginning the experiment, place the 91% isopropyl alcohol into a freezer to chill. The entire bottle may be placed in the freezer without harming the bottle or its contents. If a freezer is not available, place alcohol bottle in an ice-bath at least...
Name: Date: Recitation Ass ignment: CO2 extraction of Eugenal Due October 27 at 5:00 pm Which...
Name: Date: Recitation Ass ignment: CO2 extraction of Eugenal Due October 27 at 5:00 pm Which one of the following two compounds would you expect to have a hig her solubility in 1. iquid CO, Explain your reasoning in a few sentences: Нс. сн Capsaicin Cafleine Explain why percent recovery is utilized in the CO, extraction of Eugenol as appased to a thearetical yield. Limit yau rself to 1-3 sentences 2 Hасо, List 3 signals that should be cbserved in...
We used the technique of extraction in this lab. After extraction, what was left in solution?...
We used the technique of extraction in this lab. After extraction, what was left in solution? Why is it acceptable for those chemicals to be left to sit for a week? Think about the possible reactions that would or would not occur during the week? In our extraction, we used NaCl solution. Why did we choose to use that vs. water? In this experiment, we did a Fischer Esterification using excess acetic acid, isopentyl alcohol, and sulfuric acid to form...
The time it takes for the DNA to reach the top what would the dependent, independent,...
The time it takes for the DNA to reach the top what would the
dependent, independent, and controlled variables
be?
Method 1. Remove the calyx (green leafy end) of the strawberry 2. Insert the strawberry into a zip lock bag and tightly seal the zip lock bag afterwards 3. Use your fingers to mash the strawberry until there aren't any large pieces remaining 4. Place a strainer on top a beaker and transfer the strawberry mixture into the beaker through...
The time it takes for the DNA to reach the top what would the dependent, independent,...
The time it takes for the DNA to reach the top
what would the dependent, independent, and controlled variables
be? Please explain thank you
Method 1. Remove the calyx (green leafy end) of the strawberry 2. Insert the strawberry into a zip lock bag and tightly seal the zip lock bag afterwards 3. Use your fingers to mash the strawberry until there aren't any large pieces remaining 4. Place a strainer on top a beaker and transfer the strawberry mixture...
lab:extraction of DNA from onion tissue, and strawberries Do Onions, strawberries and Bananas Have ONA? EDVOKIE...
lab:extraction of DNA from onion tissue, and
strawberries
Do Onions, strawberries and Bananas Have ONA? EDVOKIE *5-75 Study Questions 1. Describe the appearance of the isolated DNA. 2. What is a nucleus? 3. What are nucleotides? 4. What are chromosomes? 5. What does cell lysis mean? 6. Why did the alcohol layer on top of the DNA solution? 7. Why was it important not to mix the alcohol and DNA solutions? 8. What properties of DNA allow spooling? 9. What...
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
look over the expeirment instructions in photos 1-3 then
answer questions 1-4 in the last photo
Exercise 1 Extraction of DNA In this exercise, you will extract and observe DNA from split peas. Note: The night before beginning the experiment, place the 91% isopropyl alcohol into a freezer to chill. The entire bottle may be placed in the freezer without harming the bottle or its contents. If a freezer is not available, place alcohol bottle in an ice-bath at least...
Name: Date: Recitation Ass ignment: CO2 extraction of Eugenal Due October 27 at 5:00 pm Which one of the following two compounds would you expect to have a hig her solubility in 1. iquid CO, Explain your reasoning in a few sentences: Нс. сн Capsaicin Cafleine Explain why percent recovery is utilized in the CO, extraction of Eugenol as appased to a thearetical yield. Limit yau rself to 1-3 sentences 2 Hасо, List 3 signals that should be cbserved in...
The time it takes for the DNA to reach the top what would the
dependent, independent, and controlled variables
be?
Method 1. Remove the calyx (green leafy end) of the strawberry 2. Insert the strawberry into a zip lock bag and tightly seal the zip lock bag afterwards 3. Use your fingers to mash the strawberry until there aren't any large pieces remaining 4. Place a strainer on top a beaker and transfer the strawberry mixture into the beaker through...
The time it takes for the DNA to reach the top
what would the dependent, independent, and controlled variables
be? Please explain thank you
Method 1. Remove the calyx (green leafy end) of the strawberry 2. Insert the strawberry into a zip lock bag and tightly seal the zip lock bag afterwards 3. Use your fingers to mash the strawberry until there aren't any large pieces remaining 4. Place a strainer on top a beaker and transfer the strawberry mixture...
lab:extraction of DNA from onion tissue, and
strawberries
Do Onions, strawberries and Bananas Have ONA? EDVOKIE *5-75 Study Questions 1. Describe the appearance of the isolated DNA. 2. What is a nucleus? 3. What are nucleotides? 4. What are chromosomes? 5. What does cell lysis mean? 6. Why did the alcohol layer on top of the DNA solution? 7. Why was it important not to mix the alcohol and DNA solutions? 8. What properties of DNA allow spooling? 9. What...
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