During DNA extraction . The lysis solution provides a detergent and an enzyme. What is the...
During DNA extraction
. The lysis solution provides a detergent and an enzyme. What is the enzyme included, and what is its purpose? What is a specific molecule that this enzyme acts upon and what is this molecule’s connection to DNA? Why is a detergent important in breaking open cells
Homework Answers
The enzyme included is protease (protein enzyme) is added to degrade the DNA associated proteins and other cellular proteins
the detergent is used in this process becauses it breaks down the lipids in the cell membrain and nuclei.DNA releasd after the membrains disrupter.
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During DNA extraction
. The lysis solution provides a detergent and an enzyme. What is
the...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
Please help Why is it necessary to chelate the metal ions from solution during the boiling/lysis...
Please help
Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100C? What would happen if you did not use a chelating agent such as the InstaGene matrix? 1. 2. What is needed from the cells for PCR? 3. What structures must be broken to release DNA from the cell? Why is it necessary to have a primer on each side of the DNA segment to be amplified? 4. 5. How did Ta?...
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is...
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is Inoue's transformation buffer and its role in making competent cells?
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do...
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic...
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic building block of a nucleic acid (DNA or RNA) is called a and consists of three parts. The three parts are: A. B. C. 2. Describe TWO basic differences between the structure of DNA and the structure of RNA: A. B. 3. Describe the basic difference between the function of DNA and RNA: 4. Name (write out) the names of the FOUR bases of...
Multiple Choice, Please answer all 4 What is the purpose of the neutralization solution when purifying...
Multiple Choice, Please answer all 4 What is the purpose of the neutralization solution when purifying plasmid DNA? precipitates plasmid DNA lyses the cells contains a detergent precipitates chromosomal DNA and proteins Why does the DNA bind to the purification resin? the resin is negatively charged the resin is hydrophobic the resin is positively charged the resin is neutral Why did you get colonies on plate A but not on B in the transformation, even though both plates had ampicillin?...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much of their three dimensional shape is the result of interactions between the R (variable) groups of their amino acids. The active site is the portion of the enzyme that will interact with the substrate the molecule that the enzyme acts upon. The nature and arrangement of amino acids in the active site make each enzyme specific to a substrate and to the reaction it...
A researcher has developed an experiment to measure the activity of an important liver enzyme. She...
A researcher has developed an experiment to measure the activity of an important liver enzyme. She adds the substrate for the enzymatic reaction to the dish of cells, then measures the appearance of the reaction products. The results are graphed as the amount of product on the y axis versus time on the x axis. The researcher noted four sections of the graph. For a short period of time, no products appeared (section A). In section B, the reaction rate...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
Please help
Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100C? What would happen if you did not use a chelating agent such as the InstaGene matrix? 1. 2. What is needed from the cells for PCR? 3. What structures must be broken to release DNA from the cell? Why is it necessary to have a primer on each side of the DNA segment to be amplified? 4. 5. How did Ta?...
What solution is used to isolate the DNA from the bacterial cells 7. Alkaline lysis buffer b Acidic lysis buffer RNase C d None of the above 8 What solution is used to isolate the DNA from the spin column? DNA buffer a. b. Elution buffer P1 c. d. P2 9 EcoRI restriction site is 5' GAATTC 3' and 3' CTTAAG 5 a 5' GAATTC 3' and 3' CTTAAG 5 b 5' GAATTC 3' and 3' CTTAAG 5 C. d....
Date: EXERCISE 18 uestions for Thought and Discussion Regarding the Structure of DNA: 1. The basic building block of a nucleic acid (DNA or RNA) is called a and consists of three parts. The three parts are: A. B. C. 2. Describe TWO basic differences between the structure of DNA and the structure of RNA: A. B. 3. Describe the basic difference between the function of DNA and RNA: 4. Name (write out) the names of the FOUR bases of...
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much of their three dimensional shape is the result of interactions between the R (variable) groups of their amino acids. The active site is the portion of the enzyme that will interact with the substrate the molecule that the enzyme acts upon. The nature and arrangement of amino acids in the active site make each enzyme specific to a substrate and to the reaction it...
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