1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is...
1) What is the purpose of using Lyse blue reagent for DNA extraction?
2) What is Inoue's transformation buffer and its role in making competent cells?
Homework Answers
Ans 1)
LyseBlue is a colour indicator which provides visual identification of optimum buffer mixing. It can be added to the resuspension buffer (P1) bottle before use. Also, very less amount of LyseBlue can be added to Buffer P1, enabling single plasmid preparations having visual lysis control to be performed.The reagent allows the user to monitor potential problems early in the plasmid preparation procedure.
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1) What is the purpose of using Lyse blue reagent for DNA
extraction?
2) What is...
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20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
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