Homework Answers
Strawberries are the perfect choice for a DNA extraction activity due to the fact they provide more DNA than other fruits, and they are octoploid which means they contain eight pairs of chromosomes in their nucleus. Therefore, DNA of strawberries can be easily extracted and analyzed.
During DNA extraction, soaps help to dissolve cell membrane which is lipid bilayer. Removal of lipids and proteins that surround cell and nucleus is the essential part of the protocol.
Therefore, the purpose of using soap in the DNA extraction activity using frozen strawberries is to dissolve cell membranes.
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The purpose of using soap in the DNA extraction activity using frozen strawberries is to
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is...
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is Inoue's transformation buffer and its role in making competent cells?
Onion DNA Extraction Activity Experiments Objectives
Onion DNA Extraction Activity Experiments Objectives
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1....
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
During DNA extraction . The lysis solution provides a detergent and an enzyme. What is the...
During DNA extraction . The lysis solution provides a detergent and an enzyme. What is the enzyme included, and what is its purpose? What is a specific molecule that this enzyme acts upon and what is this molecule’s connection to DNA? Why is a detergent important in breaking open cells
lab:extraction of DNA from onion tissue, and strawberries Do Onions, strawberries and Bananas Have ONA? EDVOKIE...
lab:extraction of DNA from onion tissue, and
strawberries
Do Onions, strawberries and Bananas Have ONA? EDVOKIE *5-75 Study Questions 1. Describe the appearance of the isolated DNA. 2. What is a nucleus? 3. What are nucleotides? 4. What are chromosomes? 5. What does cell lysis mean? 6. Why did the alcohol layer on top of the DNA solution? 7. Why was it important not to mix the alcohol and DNA solutions? 8. What properties of DNA allow spooling? 9. What...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
DNA Extraction Worksheet - PreLab Name Class & Section DNA Extraction Worksheet - PreLab After reading...
DNA Extraction Worksheet - PreLab
Name Class & Section DNA Extraction Worksheet - PreLab After reading "Exercise 1 DDNA Extraction" in the lab manual, answer the following questions before coming to lab. This worksheet is to be turned in at the beginning of lab. 1. (2pt) Briefly, outline the procedure for isolating DNA from your cheek cells. 2. Do all cells (except mature RBCs and gametes) contain the same DNA? 3. Do all cells use genes in the same way?...
Extraction and Recrystallization 1-2 sentence: what is the purpose of the extraction and recrystallization? 1-2 sentence...
Extraction and Recrystallization 1-2 sentence: what is the purpose of the extraction and recrystallization? 1-2 sentence the application of the extraction to the mixture and recrystallization to crude solids obtained? 1-2 sentence outcome of extraction and recrystallization?
You need 180 ng of DNA to set up a PCR. Your DNA extraction has a...
You need 180 ng of DNA to set up a PCR. Your DNA extraction has a concentration of 3ng DNA/ul. How much of my extraction do I need to use in the PCR?
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
lab:extraction of DNA from onion tissue, and
strawberries
Do Onions, strawberries and Bananas Have ONA? EDVOKIE *5-75 Study Questions 1. Describe the appearance of the isolated DNA. 2. What is a nucleus? 3. What are nucleotides? 4. What are chromosomes? 5. What does cell lysis mean? 6. Why did the alcohol layer on top of the DNA solution? 7. Why was it important not to mix the alcohol and DNA solutions? 8. What properties of DNA allow spooling? 9. What...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
DNA Extraction Worksheet - PreLab
Name Class & Section DNA Extraction Worksheet - PreLab After reading "Exercise 1 DDNA Extraction" in the lab manual, answer the following questions before coming to lab. This worksheet is to be turned in at the beginning of lab. 1. (2pt) Briefly, outline the procedure for isolating DNA from your cheek cells. 2. Do all cells (except mature RBCs and gametes) contain the same DNA? 3. Do all cells use genes in the same way?...
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