genomic DNA extraction proticol queations 1- why is sodium Azide added in wash buffer 2 2-what...
Homework Answers
- sodium azide is used in wash buffer to prevent microbial contamination in the solution, which might interfere with the protocol.
- During extraction of DNA, there is one step where the solution is centrifuged so that DNA gets attached to the membrane. After his step elution buffer is added so that the isolated DNA is eluted out (i.e., isolated) from the membrane for further use .
- the column is spuned one more time even though there is no liquid inside. It is done so that whatever liquid (buffer) is in the column, it will elute out and it's always seen that there's some buffer left in the column which if not eluted out interferes with the procedure as we don't get pure DNA
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genomic DNA extraction proticol queations
1- why is sodium Azide added in wash buffer 2
2-what...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is...
1) What is the purpose of using Lyse blue reagent for DNA extraction? 2) What is Inoue's transformation buffer and its role in making competent cells?
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1....
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
Pre-laboratory Questions 1. Although it is a less efficient solvent for this extraction, why is 2-propanol...
Pre-laboratory Questions 1. Although it is a less efficient solvent for this extraction, why is 2-propanol used in this extraction rather than chloroform or methylene chloride? 2. What is the purpose of adding sodium carbonate to the aqueous solution? Be the concentration of sodium carbonate incest, it undergoes hydolysis to form sodun hydroride 3. Why it is necessary to add sodium chloride to the water before the caffeine is extracted with 2-propanol?
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic...
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic DNA in the last Lab A. Why is it necessary to "purify" the EcoRl-cut Yeast DNA before proceeding to the DNA "cloning" steps we are about to do today? (1 pt) B. Which major type(s) of macromolecule within the restriction digest/binding buffer (PB) mixture bind(s) to the resin in the Qiagen "spin column," and which type(s) flow(s) through? (0.5 ptea)
Laboratory 34 Report Sheet - Lab 34 B. Extraction of DNA B.2 Appearance under a microscope DNA Source B.1 Texture and Appearance Questions and Problems Q.1 Why is it necessary to heat the DNA so...
Laboratory 34 Report Sheet - Lab 34 B. Extraction of DNA B.2 Appearance under a microscope DNA Source B.1 Texture and Appearance Questions and Problems Q.1 Why is it necessary to heat the DNA source and buffer mixture? Laboratory 34 Transfer the DNA from the glass rod to a paper towel and let et 17 dry. 18 Observe the DNA obtained by other student teams with ditferent DNA sources, or repeat the procedure above with another DNA souroe. Check when...
Post-Lab Report Extraction of Caffeine from Tea Leaves 1. What is liquid-liquid extraction? Explain its principle...
Post-Lab Report Extraction of Caffeine from Tea Leaves
1. What is liquid-liquid extraction? Explain its principle and show illustrations. 2. What are alkaloids and xanthines? 3. Aside from being a natural stimulant, what are the other effects of caffeine in the human body? 4. What are tannins? What are the manifestations of tannins in tea? why is it necessary to boil the solution when extracting the components from the tea leaves? 6. What can be done to avoid tannins from...
1) What happens to DNA synthesis when a dideoxyribonucleoside triphosphate ( ddNTP) is added to a...
1) What happens to DNA synthesis when a dideoxyribonucleoside triphosphate ( ddNTP) is added to a growing DNA chain ( rather than the normal deoxyribonucleoside triphosphate) ? 2) About how many copies of DNA are made after 10 cycle of PCR ? 3) What are we doing when we amplify a particular DNA segment ? 4) How are DNA fragments stored in a cDNA library ? 5) What is the basic purpose of a DNA microarray ? Please answer all...
Based on this passage what is the amount of ether for extraction, brine amount and sodium...
Based on this passage what is the amount of ether for
extraction, brine amount and sodium sulfate amount required. Please
show detail calculations if possible!
acetoacetate esters. The (S)-products are obtained. Many organisms contain enzymes that will catalyse the asymmetric reduction of some ketones. Recently, some chemists in France have shown that carrots are a good reducing agent (although brinjal, cucumber and radish are not). 13 23.11919 Biotransformation:/Reduction using Yeast Dissolve sucrose (23 g) and disodium hydrogen phosphate (150 mg)...
Name Date 5) What is the first step in the DNA extraction process? 6) What cells are used in DNA extraction? 7) Ust the four steps used to purify the DNA: a. b. d. What is the purpose of this and 8) What does the term "Lysis" mean? solution? What two chemicals does it contain? 9) What does the detergent do to the cell? What does the proteinese K do? 10) What do you think the purpose of the warm...
20- In cheek DNA extraction procedure, what is the purpose of heating a. To speed up the DNA precipitation b. To hydrolyze protein content c. To lysis cell d. To separate DNA fragments 21- What is the reason of using Taq DNA Polymerase in most of the PCR reactions. a. Taq polymerase is sensitive to high temperature b. Taq is very cheep to produce c. Taq can tolerate DNA denaturation temperature d. a, and b Assay questions 5 points each...
DNA Extraction Watch DNA extraction video DNA Modeling Post-laboratory questions: New lab manual page 44 1. Why do we use a commercially grown fruit in this case strawberries) for our DNA extraction? 2. What does the detergent accomplish in a DNA extract? 3. What does the salt accomplish in a DNA extract? 4. What does the addition of rubbing alcohol isopropanol) accomplish in the DNA extract? 5. Why does the solution become viscous after the addition of shampoo (detergent)? Students...
Pre-laboratory Questions 1. Although it is a less efficient solvent for this extraction, why is 2-propanol used in this extraction rather than chloroform or methylene chloride? 2. What is the purpose of adding sodium carbonate to the aqueous solution? Be the concentration of sodium carbonate incest, it undergoes hydolysis to form sodun hydroride 3. Why it is necessary to add sodium chloride to the water before the caffeine is extracted with 2-propanol?
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic DNA in the last Lab A. Why is it necessary to "purify" the EcoRl-cut Yeast DNA before proceeding to the DNA "cloning" steps we are about to do today? (1 pt) B. Which major type(s) of macromolecule within the restriction digest/binding buffer (PB) mixture bind(s) to the resin in the Qiagen "spin column," and which type(s) flow(s) through? (0.5 ptea)
Laboratory 34 Report Sheet - Lab 34 B. Extraction of DNA B.2 Appearance under a microscope DNA Source B.1 Texture and Appearance Questions and Problems Q.1 Why is it necessary to heat the DNA source and buffer mixture? Laboratory 34 Transfer the DNA from the glass rod to a paper towel and let et 17 dry. 18 Observe the DNA obtained by other student teams with ditferent DNA sources, or repeat the procedure above with another DNA souroe. Check when...
Post-Lab Report Extraction of Caffeine from Tea Leaves
1. What is liquid-liquid extraction? Explain its principle and show illustrations. 2. What are alkaloids and xanthines? 3. Aside from being a natural stimulant, what are the other effects of caffeine in the human body? 4. What are tannins? What are the manifestations of tannins in tea? why is it necessary to boil the solution when extracting the components from the tea leaves? 6. What can be done to avoid tannins from...
Based on this passage what is the amount of ether for
extraction, brine amount and sodium sulfate amount required. Please
show detail calculations if possible!
acetoacetate esters. The (S)-products are obtained. Many organisms contain enzymes that will catalyse the asymmetric reduction of some ketones. Recently, some chemists in France have shown that carrots are a good reducing agent (although brinjal, cucumber and radish are not). 13 23.11919 Biotransformation:/Reduction using Yeast Dissolve sucrose (23 g) and disodium hydrogen phosphate (150 mg)...
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