Question

acetoacetate esters. The (S)-products are obtained. Many organisms contain enzymes that will catalyse the asymmetric reduction of some ketones. Recently, some chemists in France have shown that carrots are a good reducing agent (although brinjal, cucumber and radish are not). 13 23.11919 Biotransformation:/Reduction using Yeast Dissolve sucrose (23 g) and disodium hydrogen phosphate (150 mg) in tap water (85 ml). Clamp the flask in a thermostatted water bath at 35°C. Add yeast (5 g), then swirl the flask. During the next, 15 minutes watch what happens, then add ethyl acetoacetate (1.5 g). Leave the flask at 35°C for one week! Clearly label your flask with your name, matric number, fume cupboard number and lab day (Thurs/Fri). Unlabeled flasks will be removed! $1293 Filter the liquid through celite, washing with ether. Do not swirl the flask or disturb the yeast layer at the bottom until the very end of the filtration process. If you do swirl it, the yeast will clog the filter, your filtration will be very, very slow and you will have to miss lunch. Separate the two layers and extract the aqueous layer again with ether. Wash the combined organic layers with brine, and dry over Na SO. After 15 minutes, filter off the sodium sulfate and evaporate the ether on the 1629kFotavap.You should obtain a clear oil. It may smell yeasty. VE1S 47 Suggestion: Weigh the sucrose directly into a flask. Measure out the water using a measuring cylinder. Weigh out the ethyl acetoacetate and add by pipet. Reduction using Sodium Borohydride Weigh ethyl acetoacetate (1.5 g) into a round bottomed flask. Add ethanol (15 anonatio stir bar and fit the flask with a drving tube

Based on this passage what is the amount of ether for extraction, brine amount and sodium sulfate amount required. Please show detail calculations if possible!

Answer 1

1)Ether amount:

Here ether is used as a solvent.There is no limit of ether quantity it's depends on solublity of our product. It is a liquid liquid extraction nearly 4 to 5 times of ether used.

Example: if a product is solid,take10 gr solid it's soluble in eather 40 gr, so we use 40 gr eather

2) Brine amount:

Here brine (Nacl solution) washing is used to remove undissolved salts in ether organic layer. There is no limit of brine quantity, approximately half quantity to the ether organic layer.

3) Sodiumsulfate amount:

Here sodiumsulfate used as a drying agent.when Na2So4 is react with water it forms Na2So4 10H2O,(Deca hydrate).

It can remove water traces from ether organic layer. Approximately 10 percent to the ether organic layer.

Example: If our organic layer is 100 gr , you can use 10 gr of Sodiumsulfate.

Thank you