Question

Name 1. Safety (10 points) Personal protective equipment (PPE) should be worn to prevent possible harm. PPE includes gloves, eye protection, and protective clothing such as lab coats. The need for PPE is dependent upon the nature and quantity of the materials in use and shall be based on risk assessment. Describe the proper safety protocol in the event that a student accidently gets burned on his or her finger using hotplate 2. Column Chromatography (10 points) 1. Column chromatography is based on the principle of 2. What is the factor responsible for the separation in column chromatography? 3. The compo nents of the mixture in column chromatography are eluted in order of 4. The elution power of a solvent is determined by 3. Lab calculations (10 points) NaOH Gualacol Gualfenesin In the reaction shown above, 0.55 mL of guaiacol was reacted with 1 mL of 3-chloro-1,2-propnae diol. What is the limiting reagent of this reaction? (density of guaiacol 1) 1.1g/mL)

Answer 1

1으 ① Column cheomatogsaphy 3t is based on the Princile of that Atatienay phate ix placed In navow coloum thsough which a mobile phase movet undox influence of guavituoxbsessuxe. 3n this indvidual Components of mixtuwe move uoith ditont rates , louver a intty and adsorption to Statonang phase move eluted out fist whide uoith adsorption move o travel Slovoeo Based on adsorponm

Restonsible for sepauation m column chromatogrph e blus olute molecule. polasu Di#ounce in po atuty de telun İneg its elution Yadu,

axe eluted in osden of Highew he fiit molecule eluted Sten uohta mined by ovetal polaoity, natwe Atahonasw phaie 4)