Question

Determine the Rf value of the substances A, B, and C on the TLC shown. Show your calculation!

4. Determine the Rf value of the substances A, B, and Con the TLC shown. Show your calculation! (6 points) solvent front LHC Origin A B C A: Bf B: Rf C: Rf

What will happen if the applied spot is lower than the level of the developing liquid?

What will be the result of applying too much compound on a TLC plate?

What is the reason that the mobile phase travels up the silica gel plate (TLC plate)?

Answer 1

"The following diagram represents TLc (thin layer chromatography). In TLC R, can calculatcd using the following formula. R_t = Distance travailed by solute pigment from origin/Distance travelled by solvent front from origin For A, R_t = Distance travailed by solute, A from origin/Distance travelled by solvent front from origin For B, R_t = Distance travailed bv solute .B from origin/Distance travelled by solvent front from origin For C, R_t = Distance travailed bv solute .C from origin/Distance travelled by solvent front from origin In TLC, Rf is the ratio of distance moved by solute from origin to distance moved by solvent from origin. i) If the applied spot is lower than developing liquid then Rf is minimum, ii) If applying the too much of compound on TLC, the identification of s