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2. The table shows the kinetic data for a reaction catalyzed by an enzyme under the following conditions: in the absence of an inhibitor, and in the presence of two different inhibitors, (1) and (2) each at a concentration of 10 mM. Assume the total enzyme concentration, [Elo, is the same for all reactions and the enzyme obeys the Michaelis-Menten mechanism In the presence of presence of 10 mM inhibitor 1 inhibitor2 In the 10 mM No inhibitor mM 2.5 4 6.3 7.6 1.17 2.1 4 5.7 7.2 0.77 1.25 10 2.5 20 2.86 Find KM and Vmax for the enzyme. For each inhibitor, determine the type of inhibition and KI and/or KI What additional information would be required to calculate the turnover number of the enzyme?

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an lo 0. 28-02 0, 6-0.4 o.0gnumloei qen/.mtg

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